KH2PO4 improves cellulase production of Irpex lacteus and Pycnoporus sanguineus

The optimization of cellulase production by Irpex lacteus and Pycnoporus sanguineus was investigated. Fractional factorial design was conducted to determine significant variables and interactions. Response surface methodology was applied through Box-Behnken design to determine the optimum level of each factor on cellulase production. The optimal conditions of culture media were (g/L): for I. lacteus CaCl2.2(H2O) 0.3, MgSO4.7(H2O) 0.3 and KH2PO4 3, while for P. sanguineus was CaCl2.2(H2O) 0.1, MgSO4.7(H2O) 0.2 and KH2PO4 9. This optimized medium improved cellulase production, especially β-glucosidase activity values for I. lacteus. KH2PO4 was found in this work to be a useful mineral compound for cellulolytic production.

Palabras clave: Cellulase , Mineral Medium , Optimization , Response Surface Methodology

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Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Atribución-NoComercial-SinDerivadas 2.5 Argentina (CC BY-NC-ND 2.5 AR)

Identificadores

URI: http://hdl.handle.net/11336/81458

DOI: http://dx.doi.org/10.1016/j.jksus.2018.07.009

URL: https://www.sciencedirect.com/science/article/pii/S1018364717309606

Colecciones

Articulos(CCT - NORDESTE)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - NORDESTE

Citación

Rodríguez, María Daniela; Alonso Paiva, Iván Mathias; Castrillo, María Lorena; Zapata, Pedro Dario; Villalba, Laura Lidia; KH2PO4 improves cellulase production of Irpex lacteus and Pycnoporus sanguineus; Elsevier Science; Journal of King Saud University - Science; 7-2018; 1-33

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