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dc.contributor.author
Castellano, Patricia Haydee
dc.contributor.author
Pérez Ibarreche, Mariana
dc.contributor.author
Longo Borges, Liliana
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Niño Arias, Fabian
dc.contributor.author
Ross, Gloria Romina
dc.contributor.author
Pereira de Martinis, Elaine Cristina
dc.date.available
2019-08-12T17:21:45Z
dc.date.issued
2018-08
dc.identifier.citation
Castellano, Patricia Haydee; Pérez Ibarreche, Mariana; Longo Borges, Liliana; Niño Arias, Fabian; Ross, Gloria Romina; et al.; Lactobacillus spp. impair the ability of listeria monocytogenes FBUNT to adhere to and invade Caco-2 cells; Springer; Biotechnology Letters; 40; 8; 8-2018; 1237-1244
dc.identifier.issn
0141-5492
dc.identifier.uri
http://hdl.handle.net/11336/81452
dc.description.abstract
The objective of this study was to evaluate the ability of Lactobacillus curvatus CRL705, CRL1532, and CRL1533 and Lactobacillus sakei CRL1613 to survive under simulated gastrointestinal conditions. Moreover, a microencapsulation approach was proposed to improve gastrointestinal survival. Finally, experiments were performed to demonstrate that Lactobacillus spp. can modulate the ability of Listeria monocytogenes FBUNT to adhere to and invade Caco-2 cells. RESULTS:Lactobacillus strains were encapsulated in alginate beads to enhance the survival of bacteria under in vitro gastrointestinal conditions. All strains hydrolyzed bile salts using chenodeoxycholic acid as a substrate and adhered to Caco-2 cells. Cell-free supernatants (CFSs) showed antimicrobial activity against L. monocytogenes as demonstrated by agar diffusion assays. The average percentages of L. monocytogenes adhesion decreased from 67.74 to 41.75 and 38.7% in the presence of 50 and 90% (v/v), respectively, for all CFSs tested. The highest concentrations of CFSs completely inhibited the L. monocytogenes invasion of Caco-2 cells.CONCLUSIONS:The studied Lactobacillus strains have protective effects against the adhesion and invasion of L. monocytogenes FBUNT. Alginate encapsulation of these bacteria improved gastrointestinal tolerance such that they could be further studied as potential probiotics against intestinal pathogenic bacteria.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Adhesion
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Invasion
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L. Monocytogenes
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Probiotic
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Biología Celular, Microbiología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Lactobacillus spp. impair the ability of listeria monocytogenes FBUNT to adhere to and invade Caco-2 cells
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-07-24T18:02:07Z
dc.identifier.eissn
1573-6776
dc.journal.volume
40
dc.journal.number
8
dc.journal.pagination
1237-1244
dc.journal.pais
Países Bajos
dc.journal.ciudad
Dordrecht
dc.description.fil
Fil: Castellano, Patricia Haydee. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
dc.description.fil
Fil: Pérez Ibarreche, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
dc.description.fil
Fil: Longo Borges, Liliana. Universidade de Sao Paulo; Brasil
dc.description.fil
Fil: Niño Arias, Fabian. Universidade de Sao Paulo; Brasil
dc.description.fil
Fil: Ross, Gloria Romina. Universidad Nacional de Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria; Argentina
dc.description.fil
Fil: Pereira de Martinis, Elaine Cristina. Universidade de Sao Paulo; Brasil
dc.journal.title
Biotechnology Letters
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1007/s10529-018-2572-x
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10529-018-2572-x
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