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dc.contributor.author
Ishizuka, Takamasa
dc.contributor.author
Kanmani, Paulraj
dc.contributor.author
Kobayashi, Hisakazu
dc.contributor.author
Miyazaki, Ayako
dc.contributor.author
Soma, Junichi
dc.contributor.author
Suda, Yoshihito
dc.contributor.author
Aso, Hisashi
dc.contributor.author
Nochi, Tomonori
dc.contributor.author
Iwabuchi, Noriyuki
dc.contributor.author
Xiao, Jin-zhong
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Saito, Tadao
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Villena, Julio Cesar
dc.contributor.author
Haruki Kitazawa
dc.date.available
2019-06-24T18:40:54Z
dc.date.issued
2016-03-29
dc.identifier.citation
Ishizuka, Takamasa; Kanmani, Paulraj; Kobayashi, Hisakazu; Miyazaki, Ayako; Soma, Junichi; et al.; Immunobiotic bifidobacteria strains modulate rotavirus immune response in porcine intestinal epitheliocytes via pattern recognition receptor signaling; Public Library of Science; Plos One; 11; 3; 29-3-2016; 1-19
dc.identifier.issn
1932-6203
dc.identifier.uri
http://hdl.handle.net/11336/78743
dc.description.abstract
In this work, we aimed to characterize the antiviral response of an originally established porcine intestinal epithelial cell line (PIE cells) by evaluating the molecular innate immune response to rotavirus (RVs). In addition, we aimed to select immunomodulatory bacteria with antiviral capabilities. PIE cells were inoculated with RVs isolated from different host species and the infective titers and the molecular innate immune response were evaluated. In addition, the protection against RVs infection and the modulation of immune response by different lactic acid bacteria (LAB) strains was studied. The RVs strains OSU (porcine) and UK (bovine) effectively infected PIE cells. Our results also showed that RVs infection in PIE cells triggered TLR3-, RIG-I- and MDA-5-mediated immune responses with activation of IRF3 and NF-κB, induction of IFN-β and up-regulation of the interferon stimulated genes MxA and RNase L. Among the LAB strains tested, Bifidobacterium infantis MCC12 and B. breve MCC1274 significantly reduced RVs titers in infected PIE cells. The beneficial effects of both bifidobacteria were associated with reduction of A20 expression, and improvements of IRF-3 activation, IFN-β production, and MxA and RNase L expressions. These results indicate the value of PIE cells for studying RVs molecular innate immune response in pigs and for the selection of beneficial bacteria with antiviral capabilities.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Public Library of Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
Immunobiotics
dc.subject
Bifidobacteria
dc.subject
Rotavirus
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Pie Cells
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Tlr3
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Otras Ciencias Biológicas
dc.subject.classification
Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Immunobiotic bifidobacteria strains modulate rotavirus immune response in porcine intestinal epitheliocytes via pattern recognition receptor signaling
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-06-03T14:35:57Z
dc.identifier.eissn
1932-6203
dc.journal.volume
11
dc.journal.number
3
dc.journal.pagination
1-19
dc.journal.pais
Estados Unidos
dc.journal.ciudad
San Francisco
dc.description.fil
Fil: Ishizuka, Takamasa. Tohoku University; Japón
dc.description.fil
Fil: Kanmani, Paulraj. Tohoku University; Japón
dc.description.fil
Fil: Kobayashi, Hisakazu. Tohoku University; Japón
dc.description.fil
Fil: Miyazaki, Ayako. National Institute of Animal Health. Viral Diseases and Epidemiology Research Division; Japón
dc.description.fil
Fil: Soma, Junichi. National Federation of Agricultural Cooperative Associations. Institute of Animal Health. Research and Development Section; Japón
dc.description.fil
Fil: Suda, Yoshihito. Miyagi University; Japón
dc.description.fil
Fil: Aso, Hisashi. Tohoku University; Japón
dc.description.fil
Fil: Nochi, Tomonori. Tohoku University; Japón
dc.description.fil
Fil: Iwabuchi, Noriyuki. Morinaga Milk Industry Co. Ltd. Food Science and Technology Institute; Japón
dc.description.fil
Fil: Xiao, Jin-zhong. Morinaga Milk Industry Co. Ltd. Food Science and Technology Institute; Japón
dc.description.fil
Fil: Saito, Tadao. Tohoku University; Japón
dc.description.fil
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón
dc.description.fil
Fil: Haruki Kitazawa. Tohoku University; Japón
dc.journal.title
Plos One
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1371/journal.pone.0152416
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0152416
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