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dc.contributor.author
Farizano, Juan Vicente
dc.contributor.author
Pescaretti, María de Las Mercedes
dc.contributor.author
Lopez, Fabian
dc.contributor.author
Fong Fu, Hsu
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Delgado, Monica Alejandra
dc.date.available
2019-05-24T14:58:20Z
dc.date.issued
2012-11
dc.identifier.citation
Farizano, Juan Vicente; Pescaretti, María de Las Mercedes; Lopez, Fabian; Fong Fu, Hsu; Delgado, Monica Alejandra; The PmrAB-inducing conditions control both lipid A remodelling and O-antigen length distribution, influencing the Salmonella Typhimurium-host interactions; American Society for Biochemistry and Molecular Biology; Journal of Biological Chemistry (online); 287; 46; 11-2012; 38778-38789
dc.identifier.issn
0021-9258
dc.identifier.uri
http://hdl.handle.net/11336/77041
dc.description.abstract
The Salmonella enterica serovar Typhimurium lipopolysaccharide consisting of covalently linked lipid A, non-repeating core oligosaccharide and the O-antigen polysaccharide, is the most exposed component of the cell envelope. Previous studies demonstrated that all of these regions act against host immunity barrier. The aim of this study was to define the role and interaction of PmrAB-dependent gene products required for the lipopolysaccharide components synthesis or modification, mainly during the Salmonella infection. The PmrAB two-component system activation promotes a remodelling of lipid A and core region by addition of 4-aminoarabinose and/or phosphoethanolamine. These PmrA-dependent activities are produced by activation of ugd, pbgPE, pmrC, cpta and pmrG transcription. In addition, under PmrA regulator activation the expression of wzzfepE and wzzst genes are induced and their products are required to determine the O-antigen chain length. We here report for the first time that Wzzst protein is necessary to maintain the balance of 4-aminoarabinose and phosphoethanolamine-lipid A modifications. Moreover, we demonstrate that the interaction of the PmrA-dependent pbgE2 and pbgE3 gene products is important for the formation of the short O-antigen region. Our results establish that PmrAB is the global regulatory system that controls lipopolysaccharide modification, leading to a coordinate regulation of 4-aminoarabinose incorporation and O-antigen chain length to respond against the host defense mechanisms.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Society for Biochemistry and Molecular Biology
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Lps
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Modifications
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Salmonella
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Typhimurium
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Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
The PmrAB-inducing conditions control both lipid A remodelling and O-antigen length distribution, influencing the Salmonella Typhimurium-host interactions
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-05-23T13:17:11Z
dc.journal.volume
287
dc.journal.number
46
dc.journal.pagination
38778-38789
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Bethesda, Maryland
dc.description.fil
Fil: Farizano, Juan Vicente. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
dc.description.fil
Fil: Pescaretti, María de Las Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
dc.description.fil
Fil: Lopez, Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
dc.description.fil
Fil: Fong Fu, Hsu. Washington University in St. Louis; Estados Unidos
dc.description.fil
Fil: Delgado, Monica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
dc.journal.title
Journal of Biological Chemistry (online)
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1074/jbc.M112.397414
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.jbc.org/content/287/46/38778.full.pdf


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