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dc.contributor.author
Kormes, Diego J.
dc.contributor.author
Corton, Eduardo
dc.date.available
2019-04-24T19:13:57Z
dc.date.issued
2009-03
dc.identifier.citation
Kormes, Diego J.; Corton, Eduardo; Development of a novel method for in vivo determination of activation energy of glucose transport across S. cerevisiae cellular membranes. A biosensor-like approach; Molecular Diversity Preservation International; Sensors; 9; 3; 3-2009; 1599-1608
dc.identifier.issn
1424-8220
dc.identifier.uri
http://hdl.handle.net/11336/74949
dc.description.abstract
Whereas biosensors have been usually proposed as analytical tools, used to investigate the surrounding media pursuing an analytical answer, we have used a biosensor-like device to characterize the microbial cells immobilized on it. We have studied the kinetics of transport and degradation of glucose at different concentrations and temperatures. When glucose concentrations of 15 and 1.5 mM were assayed, calculated activation energies were 25.2 and 18.4 kcal mol-1, respectively, in good agreement with previously published data. The opportunity and convenience of using Arrhenius plots to estimate the activation energy in metabolic-related processes is also discussed.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Molecular Diversity Preservation International
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Activation Energy
dc.subject
Arrhenius Plot
dc.subject
Biosensors
dc.subject
Glucose Transport
dc.subject
Microbial Metabolism
dc.subject
Saccharomyces Cerevisiae
dc.subject.classification
Otras Ciencias Químicas
dc.subject.classification
Ciencias Químicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Development of a novel method for in vivo determination of activation energy of glucose transport across S. cerevisiae cellular membranes. A biosensor-like approach
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-01-02T19:12:43Z
dc.journal.volume
9
dc.journal.number
3
dc.journal.pagination
1599-1608
dc.journal.pais
Suiza
dc.journal.ciudad
Basilea
dc.description.fil
Fil: Kormes, Diego J.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica. Laboratorio de Biosensores y Bioanálisis; Argentina
dc.description.fil
Fil: Corton, Eduardo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica. Laboratorio de Biosensores y Bioanálisis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Sensors
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3390/s90301599
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1424-8220/9/3/1599


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    Articulos de INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CS. EXACTAS Y NATURALES

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