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dc.contributor.author Kormes, Diego J.
dc.contributor.author Corton, Eduardo
dc.date.available 2019-04-24T19:13:57Z
dc.date.issued 2009-03
dc.identifier.citation Kormes, Diego J.; Corton, Eduardo; Development of a novel method for in vivo determination of activation energy of glucose transport across S. cerevisiae cellular membranes. A biosensor-like approach; Molecular Diversity Preservation International; Sensors; 9; 3; 3-2009; 1599-1608
dc.identifier.issn 1424-8220
dc.identifier.uri http://hdl.handle.net/11336/74949
dc.description.abstract Whereas biosensors have been usually proposed as analytical tools, used to investigate the surrounding media pursuing an analytical answer, we have used a biosensor-like device to characterize the microbial cells immobilized on it. We have studied the kinetics of transport and degradation of glucose at different concentrations and temperatures. When glucose concentrations of 15 and 1.5 mM were assayed, calculated activation energies were 25.2 and 18.4 kcal mol-1, respectively, in good agreement with previously published data. The opportunity and convenience of using Arrhenius plots to estimate the activation energy in metabolic-related processes is also discussed.
dc.format application/pdf
dc.language.iso eng
dc.publisher Molecular Diversity Preservation International
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject ACTIVATION ENERGY
dc.subject ARRHENIUS PLOT
dc.subject BIOSENSORS
dc.subject GLUCOSE TRANSPORT
dc.subject MICROBIAL METABOLISM
dc.subject SACCHAROMYCES CEREVISIAE
dc.subject.classification Otras Ciencias Químicas
dc.subject.classification Ciencias Químicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Development of a novel method for in vivo determination of activation energy of glucose transport across S. cerevisiae cellular membranes. A biosensor-like approach
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2019-01-02T19:12:43Z
dc.journal.volume 9
dc.journal.number 3
dc.journal.pagination 1599-1608
dc.journal.pais Suiza
dc.journal.ciudad Basilea
dc.description.fil Fil: Kormes, Diego J.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica. Laboratorio de Biosensores y Bioanálisis; Argentina
dc.description.fil Fil: Corton, Eduardo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica. Laboratorio de Biosensores y Bioanálisis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title Sensors
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3390/s90301599
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1424-8220/9/3/1599
dc.conicet.fuente individual


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  • Articulos(IQUIBICEN) [453]
    Articulos de INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CS. EXACTAS Y NATURALES

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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)