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dc.contributor.author Milanesi, Lorena Magdalena
dc.contributor.author Vasconsuelo, Andrea Anahi
dc.contributor.author Russo, Ana Josefa
dc.contributor.author Boland, Ricardo Leopoldo
dc.date.available 2019-02-18T19:20:41Z
dc.date.issued 2009-06
dc.identifier.citation Milanesi, Lorena Magdalena; Vasconsuelo, Andrea Anahi; Russo, Ana Josefa; Boland, Ricardo Leopoldo; Expression and subcellular distribution of native estrogen receptor β in murine C2C12 cells and skeletal muscle tissue; Elsevier Science Inc; Steroids; 74; 6; 6-2009; 489-497
dc.identifier.issn 0039-128X
dc.identifier.uri http://hdl.handle.net/11336/70376
dc.description.abstract We have recently described the expression and intracellular localization of ER α in murine C2C12 cells and skeletal muscle tissue. In separate studies, a protective role of 17β-estradiol against apoptosis exerted mainly at the mitochondrial level was also shown in the C2C12 muscle cell line. However, this functional evidence was in accordance with the participation of ER β. We have then here investigated the expression and subcellular distribution of native ER β in similar skeletal muscle cultured cells and tissue developed in vivo. ER β was detected by immunoblotting using specific antibodies and ligand blot analysis after subcellular fractionation. Immunolocalization was confirmed using conventional and confocal microscopy. ER β was found to a great extent in mitochondria and in lower amounts in the cytosolic fraction, differently to ER α which localizes in microsomes, cytosol, mitochondria, and also in the nucleus of muscle tissue. ER β expression was also demonstrated by RT-PCR. Finally, the mitochondrial localization of native ER β in C2C12 muscle cells was corroborated after transient transfection with specific ER β siRNAs. These data raise the possibility that the antiapoptotic action of 17β-estradiol in muscle cells may be related in part to a direct action of the hormone on mitochondria through ER β. © 2009 Elsevier Inc. All rights reserved.
dc.format application/pdf
dc.language.iso eng
dc.publisher Elsevier Science Inc
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject C2C12 MUSCLE CELLS
dc.subject ESTROGEN RECEPTOR β
dc.subject SKELETAL MUSCLE TISSUE
dc.subject SUBCELLULAR DISTRIBUTION
dc.subject.classification Otras Ciencias Biológicas
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Expression and subcellular distribution of native estrogen receptor β in murine C2C12 cells and skeletal muscle tissue
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2019-02-05T14:11:21Z
dc.journal.volume 74
dc.journal.number 6
dc.journal.pagination 489-497
dc.journal.pais Estados Unidos
dc.journal.ciudad Nueva York
dc.description.fil Fil: Milanesi, Lorena Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
dc.description.fil Fil: Vasconsuelo, Andrea Anahi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
dc.description.fil Fil: Russo, Ana Josefa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
dc.description.fil Fil: Boland, Ricardo Leopoldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
dc.journal.title Steroids
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1016/j.steroids.2009.01.005
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0039128X0900021X
dc.conicet.fuente individual


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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)