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dc.contributor.author
Wilda, Maximiliano
dc.contributor.author
Lavoria, Maria de Los Angeles
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Giraldez, Adrian Nicolas
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Franco Mahecha, Olga Lucia
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Mansilla, Florencia Celeste
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Érguiz, Matías
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Iglesias, Marcela Elvira
dc.contributor.author
Capozzo, Alejandra Victoria
dc.date.available
2019-01-21T23:19:07Z
dc.date.issued
2012-11
dc.identifier.citation
Wilda, Maximiliano; Lavoria, Maria de Los Angeles; Giraldez, Adrian Nicolas; Franco Mahecha, Olga Lucia; Mansilla, Florencia Celeste; et al.; Development and preliminary validation of an antibody filtration-assisted single-dilution chemiluminometric immunoassay for potency testing of Piscirickettsia salmonis vaccines; Academic Press Ltd - Elsevier Science Ltd; Biologicals; 40; 6; 11-2012; 415-420
dc.identifier.issn
1045-1056
dc.identifier.uri
http://hdl.handle.net/11336/68356
dc.description.abstract
Challenge with live pathogens could be substituted by serology for many veterinary diseases, however little progress has been made in the development of alternative batch vaccine potency tests for fish. This study reports the development and preliminary validation of a single-dilution filtration-assisted chemiluminometric immunoassay (SD FAL-ELISA) applied to measure anti Piscirickettsia salmonis IgM in individual or pooled serum and mucus samples. The assay was set up to test a single-dilution of the sample. Serum SD FAL-ELISA yielded a sensitivity of 90% and a specificity of 96%. SD FAL-ELISA was applied to evaluate pooled and individual samples from P. salmonis challenge assessments. Relative-light units values (RLU) obtained by SD FAL-ELISA were proportional to antibody levels in serum. RLU values obtained from pooled and individual serum samples increased with the observed relative percent survival (RPS) values, indicating a correlation between protection and specific IgM levels. Results obtained for specific IgM in mucus samples was not related to the RPS, but discriminated the vaccine that yielded high RPS (86.4%) from the others (40.9 and 54.5%). This is the first report on the development of an indirect high-throughput serological assessment for P. salmonis vaccine potency testing using both pooled or individual serum and cutaneous mucus samples.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Academic Press Ltd - Elsevier Science Ltd
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Fal-Elisa
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Piscirickettsia Salmonis
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Refinement
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Serology
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Vaccine Potency
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Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Development and preliminary validation of an antibody filtration-assisted single-dilution chemiluminometric immunoassay for potency testing of Piscirickettsia salmonis vaccines
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-01-09T17:50:47Z
dc.journal.volume
40
dc.journal.number
6
dc.journal.pagination
415-420
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: Wilda, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología ; Argentina
dc.description.fil
Fil: Lavoria, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología ; Argentina
dc.description.fil
Fil: Giraldez, Adrian Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología ; Argentina
dc.description.fil
Fil: Franco Mahecha, Olga Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina
dc.description.fil
Fil: Mansilla, Florencia Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina
dc.description.fil
Fil: Érguiz, Matías. Tecnovax S.A.; Argentina
dc.description.fil
Fil: Iglesias, Marcela Elvira. Tecnovax S.A.; Argentina
dc.description.fil
Fil: Capozzo, Alejandra Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
dc.journal.title
Biologicals
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.biologicals.2012.09.003
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1045105612001443
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