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dc.contributor.author Imperiale, Belén Rocío
dc.contributor.author Moyano, Roberto Damian
dc.contributor.author Di Giulio, A. B.
dc.contributor.author Romero, Magali Andrea
dc.contributor.author Alvarado Pinedo, María Fiorella
dc.contributor.author Santangelo, María de la Paz
dc.contributor.author Travería, Gabriel Eduardo
dc.contributor.author Morcillo, N. S.
dc.contributor.author Romano, Maria Isabel
dc.date.available 2018-12-27T17:57:02Z
dc.date.issued 2017-05
dc.identifier.citation Imperiale, Belén Rocío; Moyano, Roberto Damian; Di Giulio, A. B.; Romero, Magali Andrea; Alvarado Pinedo, María Fiorella; et al.; Genetic diversity of Mycobacterium avium complex strains isolated in Argentina by MIRU-VNTR; Cambridge University Press; Epidemiology and Infection; 145; 7; 5-2017; 1382-1391
dc.identifier.issn 0950-2688
dc.identifier.uri http://hdl.handle.net/11336/67079
dc.description.abstract Mycobacterium avium sp. avium (MAA), M. avium sp. hominissuis (MAH), and M. avium sp. paratuberculosis (MAP) are the main members of the M. avium complex (MAC) causing diseases in several hosts. The aim of this study was to describe the genetic diversity of MAC isolated from different hosts. Twenty-six MAH and 61 MAP isolates were recovered from humans and cattle, respectively. GenoType CM® and IS1311-PCR were used to identify Mycobacterium species. The IS901-PCR was used to differentiate between MAH and MAA, while IS900-PCR was used to identify MAP. Genotyping was performed using a mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) scheme (loci: 292, X3, 25, 47, 3, 7, 10, 32) and patterns (INMV) were assigned according to the MAC-INMV database (http://mac-inmv.tours.inra.fr/). Twenty-two (22/26, 84·6%) MAH isolates were genotyped and 16 were grouped into the following, INMV 92, INMV 121, INMV 97, INMV 103, INMV 50, and INMV 40. The loci X3 and 25 showed the largest diversity (D: 0·5844), and the global discriminatory index (Hunter and Gaston discriminatory index, HGDI) was 0·9300. MAP (100%) isolates were grouped into INMV 1, INMV 2, INMV 11, INMV 8, and INMV 5. The HGDI was 0·6984 and loci 292 and 7 had the largest D (0·6980 and 0·5050). MAH presented a higher D when compared with MAP. The MIRU-VNTR was a useful tool to describe the genetic diversity of both MAH and MAP as well as to identify six new MAH patterns that were conveniently reported to the MAC-INMV database. It was also demonstrated that, in the geographical region studied, human MAC cases were produced by MAH as there was no MAA found among the human clinical samples.
dc.format application/pdf
dc.language.iso eng
dc.publisher Cambridge University Press
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject DIVERSITY
dc.subject MIRU-VNTR
dc.subject MYCOBACTERIUM AVIUM COMPLEX
dc.subject.classification Salud Ocupacional
dc.subject.classification Ciencias de la Salud
dc.subject.classification CIENCIAS MÉDICAS Y DE LA SALUD
dc.title Genetic diversity of Mycobacterium avium complex strains isolated in Argentina by MIRU-VNTR
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2018-10-23T20:26:55Z
dc.journal.volume 145
dc.journal.number 7
dc.journal.pagination 1382-1391
dc.journal.pais Reino Unido
dc.journal.ciudad Cambridge
dc.description.fil Fil: Imperiale, Belén Rocío. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Moyano, Roberto Damian. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Di Giulio, A. B.. Provincia de Buenos Aires. Hospital Interzonal de Agudos ; Argentina
dc.description.fil Fil: Romero, Magali Andrea. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Clínica. Centro de Diagnóstico e Investigaciones Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Alvarado Pinedo, María Fiorella. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Clínica. Centro de Diagnóstico e Investigaciones Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Santangelo, María de la Paz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Travería, Gabriel Eduardo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Clínica. Centro de Diagnóstico e Investigaciones Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Morcillo, N. S.. Provincia de Buenos Aires. Ministerio de Salud. Hospital ; Argentina
dc.description.fil Fil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title Epidemiology and Infection
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1017/S0950268817000139
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/epidemiology-and-infection/article/genetic-diversity-of-mycobacterium-avium-complex-strains-isolated-in-argentina-by-miruvntr/5C0EF5C3EFC75989893FD6B6631E9962
dc.conicet.fuente Elsevier


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