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dc.contributor.author
Yanniccari, Marcos Ezequiel
dc.contributor.author
Gómez Lobato, María Eugenia
dc.contributor.author
Istilart, Carolina María
dc.contributor.author
Natalucci, Claudia Luisa
dc.contributor.author
Gimenez, Daniel Oscar
dc.contributor.author
Castro, Ana Maria
dc.date.available
2018-12-20T18:09:39Z
dc.date.issued
2017-10
dc.identifier.citation
Yanniccari, Marcos Ezequiel; Gómez Lobato, María Eugenia; Istilart, Carolina María; Natalucci, Claudia Luisa; Gimenez, Daniel Oscar; et al.; Mechanism of resistance to glyphosate in Lolium perenne from Argentina; Frontiers Media S. A; Frontiers in Ecology and Evolution; 5; OCT; 10-2017; 1-8
dc.identifier.issn
2296-701X
dc.identifier.uri
http://hdl.handle.net/11336/66847
dc.description.abstract
n Argentina, glyphosate resistance was reported in a Lolium perenne population after 12 years of successful herbicide use. The aim of the current paper was to put in evidence for the mechanism of glyphosate resistance of this weed. Susceptible leaves treated with different doses of glyphosate and incubated in vitro showed an accumulation of shikimic acid of around three to five times the basal level, while no changes were detected in leaves of glyphosate-resistant plants. The resistance mechanism prevents shikimate accumulation in leaves, even under such tissue-isolation conditions. The activity of the glyphosate target enzyme (EPSPS: 5-enolpyruvylshikimate-3-phosphate synthase) was quantified at different herbicide concentrations. EPSPS from resistant plants showed no difference in glyphosate-sensitivity compared to EPSPS from susceptible plants, and, accordingly, no amino acid substitution causing mutations associated with resistance were found. While the glyphosate target enzymes were equally sensitive, the basal EPSPS activity in glyphosate resistant plants was approximately 3-fold higher than the EPSPS activity in susceptible plants. This increased EPSPS activity in glyphosate resistant plants was associated with a 15-fold higher expression of EPSPS compared with susceptible plants. Therefore, the over-expression of EPSPS appears to be the main mechanism responsible for resistance to glyphosate. This mechanism has a constitutive character and has important effects on plant fitness, as recently reported.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Frontiers Media S. A
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Epsps Activity
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Epsps Overexpression
dc.subject
Glyphosate Resistance
dc.subject
Perennial Ryegrass
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Shikimate Assay
dc.subject.classification
Otras Biotecnología Agropecuaria
dc.subject.classification
Biotecnología Agropecuaria
dc.subject.classification
CIENCIAS AGRÍCOLAS
dc.title
Mechanism of resistance to glyphosate in Lolium perenne from Argentina
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-10-22T22:33:39Z
dc.journal.volume
5
dc.journal.number
OCT
dc.journal.pagination
1-8
dc.journal.pais
Suiza
dc.journal.ciudad
Lausana
dc.description.fil
Fil: Yanniccari, Marcos Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Barrow; Argentina
dc.description.fil
Fil: Gómez Lobato, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
dc.description.fil
Fil: Istilart, Carolina María. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Barrow; Argentina
dc.description.fil
Fil: Natalucci, Claudia Luisa. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
dc.description.fil
Fil: Gimenez, Daniel Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
dc.description.fil
Fil: Castro, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina. Universidad Nacional de la Plata. Facultad de Agronomia. Departamento de Sanidad Vegetal; Argentina
dc.journal.title
Frontiers in Ecology and Evolution
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.3389/fevo.2017.00123
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fevo.2017.00123
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