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dc.contributor.author
Giudice, Jimena
dc.contributor.author
Coluccio Leskow, Federico
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Arndt Jovin, Donna J.
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Jovin, Thomas M.
dc.contributor.author
Jares, Elizabeth Andrea
dc.date.available
2018-12-19T17:38:35Z
dc.date.issued
2011-03
dc.identifier.citation
Giudice, Jimena; Coluccio Leskow, Federico; Arndt Jovin, Donna J.; Jovin, Thomas M.; Jares, Elizabeth Andrea; Differential endocytosis and signaling dynamics of insulin receptor variants IR-A and IR-B; Company of Biologists; Journal of Cell Science; 124; 5; 3-2011; 801-811
dc.identifier.issn
0021-9533
dc.identifier.uri
http://hdl.handle.net/11336/66761
dc.description.abstract
Insulin signaling comprises a complex cascade of events, playing a key role in the regulation of glucose metabolism and cellular growth. Impaired response to insulin is the hallmark of diabetes, whereas upregulated insulin activity occurs in many cancers. Two splice variants of the insulin receptor (IR) exist in mammals: IR-A, lacking exon 11, and full-length IR-B. Although considerable biochemical data exist on insulin binding and downstream signaling, little is known about the dynamics of the IR itself. We created functional IR transgenes fused with visible fluorescent proteins for use in combination with biotinamido-caproyl insulin and streptavidin quantum dots. Using confocal and structured illumination microscopy, we visualized the endocytosis of both isoforms in living and fixed cells and demonstrated a higher rate of endocytosis of IR-A than IR-B. These differences correlated with higher and sustained activation of IR-A in response to insulin and with distinctive ERK1/2 activation profiles and gene transcription regulation. In addition, cells expressing IR-B showed higher AKT phosphorylation after insulin stimulation than cells expressing IR-A. Taken together, these results suggest that IR signaling is dependent on localization; internalized IRs regulate mitogenic activity, whereas metabolic balance signaling occurs at the cell membrane.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Company of Biologists
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Insulin
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Insulin Receptor Isoforms
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Programmable Array Microscope
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Quantum Dots
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Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Differential endocytosis and signaling dynamics of insulin receptor variants IR-A and IR-B
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-11-22T15:26:41Z
dc.journal.volume
124
dc.journal.number
5
dc.journal.pagination
801-811
dc.journal.pais
Reino Unido
dc.journal.ciudad
Cambridge
dc.description.fil
Fil: Giudice, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
dc.description.fil
Fil: Coluccio Leskow, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
dc.description.fil
Fil: Arndt Jovin, Donna J.. Max-Planck Institute for Biophysical Chemistry; Alemania
dc.description.fil
Fil: Jovin, Thomas M.. Max-Planck Institute for Biophysical Chemistry; Alemania. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
dc.description.fil
Fil: Jares, Elizabeth Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
dc.journal.title
Journal of Cell Science
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1242/jcs.076869
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://jcs.biologists.org/content/124/5/801


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    Articulos de INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CS. EXACTAS Y NATURALES

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