Mostrar el registro sencillo del ítem
dc.contributor.author
Roger, Magali
dc.contributor.author
Sciara, Giuliano
dc.contributor.author
Biaso, Frédéric
dc.contributor.author
Lojou, Elisabeth
dc.contributor.author
Wang, Xie
dc.contributor.author
Bauzan, Marielle
dc.contributor.author
Giudici Orticoni, Marie-Thérèse
dc.contributor.author
Vila, Alejandro Jose
dc.contributor.author
Ilbert, Marianne
dc.date.available
2018-12-13T19:03:36Z
dc.date.issued
2017-05
dc.identifier.citation
Roger, Magali; Sciara, Giuliano; Biaso, Frédéric; Lojou, Elisabeth; Wang, Xie; et al.; Impact of copper ligand mutations on a cupredoxin with a green copper center; Elsevier Science; Biochimica Et Biophysica Acta-bioenergetics; 1858; 5; 5-2017; 351-359
dc.identifier.issn
0005-2728
dc.identifier.uri
http://hdl.handle.net/11336/66444
dc.description.abstract
Mononuclear cupredoxins contain a type 1 copper center with a trigonal or tetragonal geometry usually maintained by four ligands, a cystein, two histidines and a methionine. The recent discovery of new members of this family with unusual properties demonstrates, however, the versatility of this class of proteins. Changes in their ligand set lead to drastic variation in their metal site geometry and in the resulting spectroscopic and redox features. In our work, we report the identification of the copper ligands in the recently discovered cupredoxin AcoP. We show that even though AcoP possesses a classical copper ligand set, it has a highly perturbed copper center. In depth studies of mutant's properties suggest a high degree of constraint existing in the copper center of the wild type protein and even the addition of exogenous ligands does not lead to the reconstitution of the initial copper center. Not only the chemical nature of the axial ligand but also constraints brought by its covalent binding to the protein backbone might be critical to maintain a green copper site with high redox potential. This work illustrates the importance of experimentally dissecting the molecular diversity of cupredoxins to determine the molecular determinants responsible for their copper center geometry and redox potential.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Acidithiobacillus Ferrooxidans
dc.subject
Copper
dc.subject
Cupredoxin
dc.subject
Green Copper Site
dc.subject
Metal Center
dc.subject
Metalloprotein
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Impact of copper ligand mutations on a cupredoxin with a green copper center
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-10-23T16:36:03Z
dc.journal.volume
1858
dc.journal.number
5
dc.journal.pagination
351-359
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Roger, Magali. Aix Marseille Univ; Francia
dc.description.fil
Fil: Sciara, Giuliano. Aix Marseille Univ; Francia
dc.description.fil
Fil: Biaso, Frédéric. Aix Marseille Univ; Francia
dc.description.fil
Fil: Lojou, Elisabeth. Aix Marseille Univ; Francia
dc.description.fil
Fil: Wang, Xie. Aix Marseille Univ; Francia
dc.description.fil
Fil: Bauzan, Marielle. Aix Marseille Univ; Francia
dc.description.fil
Fil: Giudici Orticoni, Marie-Thérèse. Aix Marseille Univ; Francia
dc.description.fil
Fil: Vila, Alejandro Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química Biológica. Área Biofísica; Argentina
dc.description.fil
Fil: Ilbert, Marianne. Aix Marseille Univ; Francia
dc.journal.title
Biochimica Et Biophysica Acta-bioenergetics
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0005272817300294
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1016/j.bbabio.2017.02.007
Archivos asociados