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dc.contributor.author Roger, Magali
dc.contributor.author Sciara, Giuliano
dc.contributor.author Biaso, Frédéric
dc.contributor.author Lojou, Elisabeth
dc.contributor.author Wang, Xie
dc.contributor.author Bauzan, Marielle
dc.contributor.author Giudici Orticoni, Marie-Thérèse
dc.contributor.author Vila, Alejandro Jose
dc.contributor.author Ilbert, Marianne
dc.date.available 2018-12-13T19:03:36Z
dc.date.issued 2017-05
dc.identifier.citation Roger, Magali; Sciara, Giuliano; Biaso, Frédéric; Lojou, Elisabeth; Wang, Xie; et al.; Impact of copper ligand mutations on a cupredoxin with a green copper center; Elsevier Science; Biochimica Et Biophysica Acta-bioenergetics; 1858; 5; 5-2017; 351-359
dc.identifier.issn 0005-2728
dc.identifier.uri http://hdl.handle.net/11336/66444
dc.description.abstract Mononuclear cupredoxins contain a type 1 copper center with a trigonal or tetragonal geometry usually maintained by four ligands, a cystein, two histidines and a methionine. The recent discovery of new members of this family with unusual properties demonstrates, however, the versatility of this class of proteins. Changes in their ligand set lead to drastic variation in their metal site geometry and in the resulting spectroscopic and redox features. In our work, we report the identification of the copper ligands in the recently discovered cupredoxin AcoP. We show that even though AcoP possesses a classical copper ligand set, it has a highly perturbed copper center. In depth studies of mutant's properties suggest a high degree of constraint existing in the copper center of the wild type protein and even the addition of exogenous ligands does not lead to the reconstitution of the initial copper center. Not only the chemical nature of the axial ligand but also constraints brought by its covalent binding to the protein backbone might be critical to maintain a green copper site with high redox potential. This work illustrates the importance of experimentally dissecting the molecular diversity of cupredoxins to determine the molecular determinants responsible for their copper center geometry and redox potential.
dc.format application/pdf
dc.language.iso eng
dc.publisher Elsevier Science
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject ACIDITHIOBACILLUS FERROOXIDANS
dc.subject COPPER
dc.subject CUPREDOXIN
dc.subject GREEN COPPER SITE
dc.subject METAL CENTER
dc.subject METALLOPROTEIN
dc.subject.classification Bioquímica y Biología Molecular
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Impact of copper ligand mutations on a cupredoxin with a green copper center
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2018-10-23T16:36:03Z
dc.journal.volume 1858
dc.journal.number 5
dc.journal.pagination 351-359
dc.journal.pais Países Bajos
dc.journal.ciudad Amsterdam
dc.description.fil Fil: Roger, Magali. Aix Marseille Univ; Francia
dc.description.fil Fil: Sciara, Giuliano. Aix Marseille Univ; Francia
dc.description.fil Fil: Biaso, Frédéric. Aix Marseille Univ; Francia
dc.description.fil Fil: Lojou, Elisabeth. Aix Marseille Univ; Francia
dc.description.fil Fil: Wang, Xie. Aix Marseille Univ; Francia
dc.description.fil Fil: Bauzan, Marielle. Aix Marseille Univ; Francia
dc.description.fil Fil: Giudici Orticoni, Marie-Thérèse. Aix Marseille Univ; Francia
dc.description.fil Fil: Vila, Alejandro Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química Biológica. Área Biofísica; Argentina
dc.description.fil Fil: Ilbert, Marianne. Aix Marseille Univ; Francia
dc.journal.title Biochimica Et Biophysica Acta-bioenergetics
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0005272817300294
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1016/j.bbabio.2017.02.007
dc.conicet.fuente individual


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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)