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dc.contributor.author
Taveira, Gabriel B.
dc.contributor.author
Mello, Érica O.
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Carvalho, André O.
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Regente, Mariana Clelia
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Pinedo, Marcela Lilian
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de la Canal, Laura
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Rodrigues, Rosana
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Gomes, Valdirene M.
dc.date.available
2018-11-27T14:07:47Z
dc.date.issued
2017-05
dc.identifier.citation
Taveira, Gabriel B.; Mello, Érica O.; Carvalho, André O.; Regente, Mariana Clelia; Pinedo, Marcela Lilian; et al.; Antimicrobial activity and mechanism of action of a thionin-like peptide from Capsicum annuum fruits and combinatorial treatment with fluconazole against Fusarium solani; John Wiley & Sons Inc; Biopolymers; 108; 3; 5-2017
dc.identifier.issn
0006-3525
dc.identifier.uri
http://hdl.handle.net/11336/65285
dc.description.abstract
Many Fusarium species are able to cause severe infections in plants as well as in animals and humans. Therefore, the discovery of new antifungal agents is of paramount importance. CaThi belongs to the thionins, which are cationic peptides with low molecular weights (∼5 kDa) that have toxic effects against various microorganisms. Herein, we study the mechanism of action of CaThi and its combinatory effect with fluconazole (FLC) against Fusarium solani. The mechanism of action of CaThi was studied by growth inhibition, viability, plasma membrane permeabilization, ROS induction, caspase activation, localization, and DNA binding capability, as assessed with Sytox green, DAB, FITC-VAD-FMK, CaThi-FITC, and gel shift assays. The combinatory effect of CaThi and FLC was assessed using a growth inhibition assay. Our results demonstrated that CaThi present a dose dependent activity and at the higher used concentration (50 µg mL−1) inhibits 83% of F. solani growth, prevents the formation of hyphae, permeabilizes membranes, induces endogenous H2O2, activates caspases, and localizes intracellularly. CaThi combined with FLC, at concentrations that alone do not inhibit F. solani, result in 100% death of F. solani when combined. The data presented in this study demonstrate that CaThi causes death of F. solani via apoptosis; an intracellular target may also be involved. Combined treatment using CaThi and FLC is a strong candidate for studies aimed at improved targeting of F. solani. This strategy is of particular interest because it minimizes selection of resistant microorganisms.
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application/pdf
dc.language.iso
eng
dc.publisher
John Wiley & Sons Inc
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Antimicrobial Peptides
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Fungi
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Membrane Permeability
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Synergism
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Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Antimicrobial activity and mechanism of action of a thionin-like peptide from Capsicum annuum fruits and combinatorial treatment with fluconazole against Fusarium solani
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-10-23T14:08:43Z
dc.journal.volume
108
dc.journal.number
3
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Nueva York
dc.description.fil
Fil: Taveira, Gabriel B.. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil
dc.description.fil
Fil: Mello, Érica O.. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil
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Fil: Carvalho, André O.. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil
dc.description.fil
Fil: Regente, Mariana Clelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
dc.description.fil
Fil: Pinedo, Marcela Lilian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
dc.description.fil
Fil: de la Canal, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
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Fil: Rodrigues, Rosana. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil
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Fil: Gomes, Valdirene M.. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil
dc.journal.title
Biopolymers
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://doi.wiley.com/10.1002/bip.23008
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1002/bip.23008
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