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dc.contributor.author
Gonorazky, Ana Gabriela  
dc.contributor.author
Guzzo, Maria Carla  
dc.contributor.author
Abd El Haliem, Ahmed M.  
dc.contributor.author
Joosten, Matthieu H. A. J.  
dc.contributor.author
Laxalt, Ana Maria  
dc.date.available
2018-10-17T19:19:42Z  
dc.date.issued
2016-12  
dc.identifier.citation
Gonorazky, Ana Gabriela; Guzzo, Maria Carla; Abd El Haliem, Ahmed M.; Joosten, Matthieu H. A. J.; Laxalt, Ana Maria; Silencing of the tomato phosphatidylinositol-phospholipase C2 (SLPLC2) reduces plant susceptibility to Botrytis cinerea; Wiley Blackwell Publishing, Inc; Molecular Plant Pathology; 17; 9; 12-2016; 1354-1363  
dc.identifier.issn
1464-6722  
dc.identifier.uri
http://hdl.handle.net/11336/62636  
dc.description.abstract
The tomato [Solanum lycopersicum (Sl)] phosphatidylinositol-phospholipase C (PI-PLC) gene family is composed of six members, named SlPLC1 to SlPLC6, differentially regulated on pathogen attack. We have previously shown that the fungal elicitor xylanase induces a raise of SlPLC2 and SlPLC5 transcripts and that SlPLC2, but not SlPLC5, is required for xylanase-induced expression of defense-related genes. In this work we studied the role of SlPLC2 in the interaction between tomato and the necrotrophic fungus Botrytis cinerea. Inoculation of tomato leaves with B. cinerea increases SlPLC2 transcript levels. We knocked-down the expression of SlPLC2 by virus-induced gene silencing and plant defense responses were analyzed upon B. cinerea inoculation. SlPLC2 silenced plants developed smaller necrotic lesions concomitantly with less proliferation of the fungus. Silencing of SlPLC2 resulted as well in a reduced production of reactive oxygen species. Upon B. cinerea inoculation, transcript levels of the salicylic acid (SA)-defense pathway marker gene SlPR1a were diminished in SlPLC2 silenced plants compared to non-silenced infected plants, while transcripts of the jasmonic acid (JA)-defense gene markers Proteinase Inhibitor I and II (SlPI-I and SlPI-II) were increased. This implies that SlPLC2 participates in plant susceptibility to B. cinerea.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Wiley Blackwell Publishing, Inc  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Cell Death  
dc.subject
Defense Gene  
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Jasmonic Acid  
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Necrotrophic Fungus  
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Phospholipid Signalling  
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Reactive Oxygen Species  
dc.subject
Salicylic Acid  
dc.subject.classification
Otras Ciencias Biológicas  
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Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Silencing of the tomato phosphatidylinositol-phospholipase C2 (SLPLC2) reduces plant susceptibility to Botrytis cinerea  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-10-17T17:25:44Z  
dc.journal.volume
17  
dc.journal.number
9  
dc.journal.pagination
1354-1363  
dc.journal.pais
Reino Unido  
dc.journal.ciudad
Londres  
dc.description.fil
Fil: Gonorazky, Ana Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina  
dc.description.fil
Fil: Guzzo, Maria Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina  
dc.description.fil
Fil: Abd El Haliem, Ahmed M.. Wageningen University; Países Bajos  
dc.description.fil
Fil: Joosten, Matthieu H. A. J.. Wageningen University; Países Bajos  
dc.description.fil
Fil: Laxalt, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina  
dc.journal.title
Molecular Plant Pathology  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/mpp.12365  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/mpp.12365