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dc.contributor.author
Gonorazky, Ana Gabriela
dc.contributor.author
Guzzo, Maria Carla
dc.contributor.author
Abd El Haliem, Ahmed M.
dc.contributor.author
Joosten, Matthieu H. A. J.
dc.contributor.author
Laxalt, Ana Maria
dc.date.available
2018-10-17T19:19:42Z
dc.date.issued
2016-12
dc.identifier.citation
Gonorazky, Ana Gabriela; Guzzo, Maria Carla; Abd El Haliem, Ahmed M.; Joosten, Matthieu H. A. J.; Laxalt, Ana Maria; Silencing of the tomato phosphatidylinositol-phospholipase C2 (SLPLC2) reduces plant susceptibility to Botrytis cinerea; Wiley Blackwell Publishing, Inc; Molecular Plant Pathology; 17; 9; 12-2016; 1354-1363
dc.identifier.issn
1464-6722
dc.identifier.uri
http://hdl.handle.net/11336/62636
dc.description.abstract
The tomato [Solanum lycopersicum (Sl)] phosphatidylinositol-phospholipase C (PI-PLC) gene family is composed of six members, named SlPLC1 to SlPLC6, differentially regulated on pathogen attack. We have previously shown that the fungal elicitor xylanase induces a raise of SlPLC2 and SlPLC5 transcripts and that SlPLC2, but not SlPLC5, is required for xylanase-induced expression of defense-related genes. In this work we studied the role of SlPLC2 in the interaction between tomato and the necrotrophic fungus Botrytis cinerea. Inoculation of tomato leaves with B. cinerea increases SlPLC2 transcript levels. We knocked-down the expression of SlPLC2 by virus-induced gene silencing and plant defense responses were analyzed upon B. cinerea inoculation. SlPLC2 silenced plants developed smaller necrotic lesions concomitantly with less proliferation of the fungus. Silencing of SlPLC2 resulted as well in a reduced production of reactive oxygen species. Upon B. cinerea inoculation, transcript levels of the salicylic acid (SA)-defense pathway marker gene SlPR1a were diminished in SlPLC2 silenced plants compared to non-silenced infected plants, while transcripts of the jasmonic acid (JA)-defense gene markers Proteinase Inhibitor I and II (SlPI-I and SlPI-II) were increased. This implies that SlPLC2 participates in plant susceptibility to B. cinerea.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Cell Death
dc.subject
Defense Gene
dc.subject
Jasmonic Acid
dc.subject
Necrotrophic Fungus
dc.subject
Phospholipid Signalling
dc.subject
Reactive Oxygen Species
dc.subject
Salicylic Acid
dc.subject.classification
Otras Ciencias Biológicas
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Silencing of the tomato phosphatidylinositol-phospholipase C2 (SLPLC2) reduces plant susceptibility to Botrytis cinerea
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-10-17T17:25:44Z
dc.journal.volume
17
dc.journal.number
9
dc.journal.pagination
1354-1363
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Gonorazky, Ana Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
dc.description.fil
Fil: Guzzo, Maria Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
dc.description.fil
Fil: Abd El Haliem, Ahmed M.. Wageningen University; Países Bajos
dc.description.fil
Fil: Joosten, Matthieu H. A. J.. Wageningen University; Países Bajos
dc.description.fil
Fil: Laxalt, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
dc.journal.title
Molecular Plant Pathology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/mpp.12365
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/mpp.12365


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