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Artículo

Sensitive ergotamine determination in pharmaceuticals and biological samples using cloud point preconcentration and spectrofluorimetric detection

Wang, Chien ChunIcon ; Fernandez, Liliana PatriciaIcon ; Gomez, Maria Roxana AnabelIcon
Fecha de publicación: 01/2013
Editorial: Elsevier
Revista: Analytica Chimica Acta
ISSN: 0003-2670
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Química Analítica

Resumen

A new cloud point extraction (CPE) method for ergotamine analysis using fluorimetric detection is described. Ergotamine from an aqueous solution was preconcentrated into a smaller surfactant-rich phase using nonionic surfactant polyoxyethylene(7.5)nonylphenylether (PONPE 7.5). Differently from the conventional CPE procedure in which the resulting surfactant-rich phase is diluted by a fluidificant before its analysis, in this method the fluorescence measurements were carried out directly onto the undiluted surfactant-rich phase. The high viscosity provided by the undiluted surfactant rich phase greatly improved the fluorescence emission of ergotamine, leading to a total enhancement factor of 1325. This spectral advantage plus the preconcentration factor achieved, contributed to the method sensitivity allowing the ergotamine determination at trace level concentration. Under optimal experimental conditions, a linear calibration curve was obtained from 3.81 × 10−7 to 1.10 μg mL−1, with detection and quantification limits of 0.11 and 0.38 pg mL−1, respectively. The accuracy and versatility of the present methodology were proved by analyzing ergotamine in real samples of different natures such as pharmaceuticals, urine and saliva.
Palabras clave: Ergotamine , Cloud Point Extraction , Spectrofluorimetry , Pharmaceuticals , Urine , Saliva
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Atribución-NoComercial-SinDerivadas 2.5 Argentina (CC BY-NC-ND 2.5 AR)
Identificadores
URI: http://hdl.handle.net/11336/5771
DOI: http://dx.doi.org/ 10.1016/j.aca.2013.01.023
DOI: http://dx.doi.org/10.1016/j.aca.2013.01.023
URL: http://www.sciencedirect.com/science/article/pii/S0003267013001712
Colecciones
Articulos(CCT - SAN LUIS)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SAN LUIS
Articulos(INQUISAL)
Articulos de INST. DE QUIMICA DE SAN LUIS
Citación
Wang, Chien Chun; Fernandez, Liliana Patricia; Gomez, Maria Roxana Anabel; Sensitive ergotamine determination in pharmaceuticals and biological samples using cloud point preconcentration and spectrofluorimetric detection; Elsevier; Analytica Chimica Acta; 768; 1-2013; 90-95
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