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Artículo

Proteolytic action of Lactobacillus delbrueckii subsp. bulgaricus CRL 656 reduces antigenic response to bovine β-lactoglobulin

Pescuma, MicaelaIcon ; Hebert, Elvira MariaIcon ; Rabesona, Hanitra; Drouet, Martine; Choiset, Yvan; Haertlé, Thomas; Mozzi, Fernanda BeatrizIcon ; Font, Graciela MariaIcon ; Chobert, Jean-Marc
Fecha de publicación: 15/07/2011
Editorial: Elsevier
Revista: Food Chemistry
ISSN: 0308-8146
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Alimentos y Bebidas

Resumen

The whey protein β-lactoglobulin (BLG) is highly allergenic. Lactic acid bacteria can degrade milk proteins. The capacity of Lactobacillus delbrueckii subsp. bulgaricus CRL 656 to hydrolyse the major BLG epitopes (V41-K60; Y102-R124; L149-I162) and decrease their recognition by IgE of allergic patients was evaluated. The intensity of BLG degradation was analysed by Tricine SDS-PAGE and RP-HPLC. Peptides released were identified by LC-MS/MS and the hydrolysates were tested for their capacity to inhibit IgE binding by ELISA test. L. delbrueckii subsp. bulgaricus CRL 656 degraded BLG (35%, 8 h). The sequence analysis of the released peptides indicated that this strain degraded three main BLG epitopes. BLG-positive sera (3-5 year old children) were used for testing IgE binding inhibition of BLG hydrolysates from the Lactobacillus strain. The hydrolysates were less immuno-reactive (32%) than the heated BLG. L. delbrueckii subsp. bulgaricus CRL 656 could be used for developing hypoallergenic dairy products.
Palabras clave: Β-Lactoglobulin , Allergy , Lactic Acid Bacteria , Lactobacillus
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/57250
DOI: http://dx.doi.org/10.1016/j.foodchem.2011.01.029
URL: https://www.sciencedirect.com/science/article/pii/S0308814611000914
Colecciones
Articulos(CERELA)
Articulos de CENTRO DE REFERENCIA PARA LACTOBACILOS (I)
Citación
Pescuma, Micaela; Hebert, Elvira Maria; Rabesona, Hanitra; Drouet, Martine; Choiset, Yvan; et al.; Proteolytic action of Lactobacillus delbrueckii subsp. bulgaricus CRL 656 reduces antigenic response to bovine β-lactoglobulin; Elsevier; Food Chemistry; 127; 2; 15-7-2011; 487-492
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