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dc.contributor.author
Isaguirre, Andrea Celeste  
dc.contributor.author
Acosta, Maria Gimena  
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Cerutti, Estela Soledad  
dc.contributor.author
Fernandez, Liliana Patricia  
dc.date.available
2018-08-15T18:53:21Z  
dc.date.issued
2016-11  
dc.identifier.citation
Isaguirre, Andrea Celeste; Acosta, Maria Gimena; Cerutti, Estela Soledad; Fernandez, Liliana Patricia; New flow injection method for quality control of dietary supplements containing L-carnitine using extraction mediated by sodium taurodeoxycholate coacervate coupled to molecular fluorescence; Elsevier Science; Microchemical Journal; 129; 11-2016; 268-273  
dc.identifier.issn
0026-265X  
dc.identifier.uri
http://hdl.handle.net/11336/55702  
dc.description.abstract
Carnitine (CAR) is an essential component of the tissue of animals, higher plants and many microorganisms; it plays an important role during the metabolism of the human body. CAR transfers fatty acids to the place of burning-mitochondria and aids in the transformation of fats into energy and in this way supports overweight reduction and immediate physical performance, increases resistance from physical load and protects heart from overload. In this study, a new methodology for the determination of L-CAR by molecular fluorescence is described, after derivatization with a fluorescence active substance, 9-fluorenylmethyl chloroformate. An on-line coacervation process was used in order to isolate the analyte of the complex matrix. The developed methodology was successfully applied to the control of carnitine content in dietary supplements. The detection limit and quantification obtained were 2.4 × 10− 8 mol L− 1 and 7.30 × 10− 8 mol L− 1; respectively. Recoveries between 82 and 103% were obtained. At optimized working conditions, the sample throughput was 30 samples h− 1 (without considering the required time for L-CAR derivatization). Thus short analysis and very low running costs compared with the LC/MS conventional methodology can be cited as the main advantages of the on-line coacervation with fluorescence detection proposed methodology.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier Science  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Bile Salt Coacervation  
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Flow Injection Analysis  
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Fluorescence Detection  
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L-Carnitine  
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Pharmaceutical Quality Control  
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Otras Ciencias Químicas  
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Ciencias Químicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
New flow injection method for quality control of dietary supplements containing L-carnitine using extraction mediated by sodium taurodeoxycholate coacervate coupled to molecular fluorescence  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-08-15T13:58:08Z  
dc.journal.volume
129  
dc.journal.pagination
268-273  
dc.journal.pais
Países Bajos  
dc.journal.ciudad
Amsterdam  
dc.description.fil
Fil: Isaguirre, Andrea Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina  
dc.description.fil
Fil: Acosta, Maria Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina  
dc.description.fil
Fil: Cerutti, Estela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química. Área de Química Analítica; Argentina  
dc.description.fil
Fil: Fernandez, Liliana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química. Área de Química Analítica; Argentina  
dc.journal.title
Microchemical Journal  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.microc.2016.06.025  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0026265X16301126