Artículo
Effect of an Ilex paraguariensis (yerba mate) extract on infarct size in isolated rat hearts: The mechanisms involved
González Arbeláez, Luisa Fernanda
; Fantinelli, Juliana Catalina
; Ciocci Pardo, Alejandro
; Caldiz, Claudia Irma
; Ríos, José Luis; Schinella, Guillermo Raúl; Mosca, Susana Maria
Fecha de publicación:
02/2016
Editorial:
Royal Society of Chemistry
Revista:
Food and Function
ISSN:
2042-6496
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Tea made from Ilex paraguariensis (IP) dried and minced leaves is a beverage widely consumed by large populations in South America as a source of caffeine (stimulant action) and for its medicinal properties. However, there is little information about the action of IP on the myocardium in the ischemia-reperfusion condition. Therefore, the objective of this study was to examine the effects of an aqueous extract of IP on infarct size in a model of regional ischemia. Isolated rat hearts were perfused by the Langendorff technique and subjected to 40 min of coronary artery occlusion followed by 60 min of reperfusion (ischemic control hearts). Other hearts received IP 30 μg mL-1 during the first 10 min of reperfusion in the absence or presence of lG-nitro-l-arginine methyl ester [l-NAME, a nitric oxide synthase (NOS) inhibitor]. The infarct size was determined by triphenyltetrazolium chloride (TTC) staining. Post-ischemic myocardial function and coronary perfusion were also assessed. Cardiac oxidative damage was evaluated by using the thiobarbituric acid reactive substance (TBARS) concentration and the reduced glutathione (GSH) content. To analyze the mechanisms involved, the expressions of phosphorylated forms of eNOS and Akt were measured. In isolated mitochondria the Ca2+-induced mitochondrial permeability transition pore (mPTP) opening was determined. IP significantly decreased the infarct size and improved post-ischemic myocardial function and coronary perfusion. TBARS decreased, GSH was partially preserved, the levels of P-eNOS and P-Akt increased and mPTP opening diminished after IP addition. These changes were abolished by l-NAME. Therefore, our data demonstrate that acute treatment with IP only during reperfusion was effective in reducing myocardial post-ischemic alterations. These actions would be mediated by a decrease of mitochondrial permeability through IP-activated Akt/eNOS-dependent pathways.
Palabras clave:
Ilex Paraguariensis
,
Ischemia-Reperfusion
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Articulos(CIC)
Articulos de CENTRO DE INVEST.CARDIOVASCULARES (I)
Articulos de CENTRO DE INVEST.CARDIOVASCULARES (I)
Citación
González Arbeláez, Luisa Fernanda; Fantinelli, Juliana Catalina; Ciocci Pardo, Alejandro; Caldiz, Claudia Irma; Ríos, José Luis; et al.; Effect of an Ilex paraguariensis (yerba mate) extract on infarct size in isolated rat hearts: The mechanisms involved; Royal Society of Chemistry; Food and Function; 7; 2; 2-2016; 816-824
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