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dc.contributor.author
Padula, Gisel
dc.contributor.author
Ponzinibbio, Maria Virginia
dc.contributor.author
Seoane, Analia Isabel
dc.date.available
2018-08-01T22:42:09Z
dc.date.issued
2016-08
dc.identifier.citation
Padula, Gisel; Ponzinibbio, Maria Virginia; Seoane, Analia Isabel; Possible radio-protective effect of folic acid supplementation on low dose ionizing radiation-induced genomic instability in vitro; National Institute of Science Communication; Indian Journal of Experimental Biology; 54; 8; 8-2016; 537-543
dc.identifier.issn
0019-5189
dc.identifier.uri
http://hdl.handle.net/11336/53861
dc.description.abstract
Ionizing radiation induces DNA damage through the production of single-strand breaks, double-strand breaks and reactive oxygen species. Folic acid (FA) can prevent DNA damage. We hypothesized that in vitro supplementation with FA will decrease the sensitivity of cells to genetic damage induced by low dose of ionizing radiation. Annexin V, comet and micronucleus assays were performed in cultured CHO cells. After 6 days of pre-treatment with 0, 100, 200 or 300 nM FA cultures were exposed to radiation (100 mSv). Two un-irradiated controls were executed (0 and 100 nM FA). Data were statistically analyzed with X2-test and linear regression analysis (P 0.05). We observed a significant decrement in the frequency of apoptotic cells as the folic acid concentration increases (P < 0.05). The same trend was observed when analyzing DNA damage and chromosomal instability (P < 0.05 for 300 nM). For the linear regression analysis, only micronuclei frequencies shows significant differences (R2 = 94.04; P < 0.01). Our results indicate that folate supplementation has an important impact on genetic damage and should be taken into account when studying the effect of low dose radiation in environmental or occupational exposure.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
National Institute of Science Communication
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Low Dose of Ionizing Radiation
dc.subject
Radiation Effects
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Folic Acid Supplementation
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Dna Damage
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Apoptosis
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Cho Cells
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Chromosome Instability
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Occupational/Enviromental Hazards
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Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Possible radio-protective effect of folic acid supplementation on low dose ionizing radiation-induced genomic instability in vitro
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-06-11T14:28:56Z
dc.identifier.eissn
0975-1009
dc.journal.volume
54
dc.journal.number
8
dc.journal.pagination
537-543
dc.journal.pais
India
dc.journal.ciudad
Nueva Delhi
dc.description.fil
Fil: Padula, Gisel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - La Plata. Instituto de Genética Veterinaria Ing. Fernando Noel Dulout; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; Argentina
dc.description.fil
Fil: Ponzinibbio, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - La Plata. Instituto de Genética Veterinaria Ing. Fernando Noel Dulout; Argentina
dc.description.fil
Fil: Seoane, Analia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - La Plata. Instituto de Genética Veterinaria Ing. Fernando Noel Dulout; Argentina
dc.journal.title
Indian Journal of Experimental Biology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://nopr.niscair.res.in/handle/123456789/35146
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