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dc.contributor.author
Massaldi, Hugo Alberto
dc.contributor.author
Bessio, M. Inés
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Suárez, Norma
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Texeira, Esther
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Rossi, Silvina
dc.contributor.author
Ferreira, Fernando
dc.date.available
2018-08-01T20:44:43Z
dc.date.issued
2010-01
dc.identifier.citation
Massaldi, Hugo Alberto; Bessio, M. Inés; Suárez, Norma; Texeira, Esther; Rossi, Silvina; et al.; Features of bacterial growth and polysaccharide production of Streptococcus pneumoniae serotype 14; Wiley; Biotechnology and Applied Biochemistry; 55; 1; 1-2010; 37-43
dc.identifier.issn
0885-4513
dc.identifier.uri
http://hdl.handle.net/11336/53838
dc.description.abstract
The effect of several cultivation conditions on the kinetics of bacterial growth and polysaccharide production of Streptococcus pneumoniae serotype 14 was studied. The presence in the supernatant of serotype-specific CPS (capsular polysaccharide) during growth was followed by size-exclusion HPLC and, in parallel, confirmed by using a specific latex reagent. The agitation level did not affect the production behaviour, whereas pH maintenance above 6 strongly enhanced both growth and CPS production throughout the cultivation period in flasks. Production of high-molecular-mass polysaccharide was found to be maximal between 5 and 6 h of cultivation, at the end of the exponential phase. By laser light scattering, 90% of this purified CPS product showed a Mw (molecular mass) range from 350 to 1500 kDa, with an average Mw of 921 kDa. Extending the culture to 24 h gave rise to a clear shift of the Mw distribution of the polysaccharide to values lower than 100 kDa. These findings may have strong implications for the large-scale manufacture of the polysaccharide and the associated conjugate vaccine.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Capsular Polysaccharide
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Capsule
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Fermentation
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Pneumoccocus
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Streptococcus Pneumoniae
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Vaccine
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Biotecnología Industrial
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Biotecnología Industrial
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INGENIERÍAS Y TECNOLOGÍAS
dc.title
Features of bacterial growth and polysaccharide production of Streptococcus pneumoniae serotype 14
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-07-30T15:43:49Z
dc.journal.volume
55
dc.journal.number
1
dc.journal.pagination
37-43
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Massaldi, Hugo Alberto. Universidad de la Republica Instituto de Higiene; Uruguay. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Bessio, M. Inés. Universidad de la Republica Instituto de Higiene; Uruguay
dc.description.fil
Fil: Suárez, Norma. Universidad de la Republica Instituto de Higiene; Uruguay
dc.description.fil
Fil: Texeira, Esther. Universidad de la Republica Instituto de Higiene; Uruguay
dc.description.fil
Fil: Rossi, Silvina. Universidad de la Republica Instituto de Higiene; Uruguay
dc.description.fil
Fil: Ferreira, Fernando. Universidad de la Republica Instituto de Higiene; Uruguay
dc.journal.title
Biotechnology and Applied Biochemistry
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1042/BA20090218
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