Artículo
Features of bacterial growth and polysaccharide production of Streptococcus pneumoniae serotype 14
Massaldi, Hugo Alberto
; Bessio, M. Inés; Suárez, Norma; Texeira, Esther; Rossi, Silvina; Ferreira, Fernando
Fecha de publicación:
01/2010
Editorial:
Wiley
Revista:
Biotechnology and Applied Biochemistry
ISSN:
0885-4513
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The effect of several cultivation conditions on the kinetics of bacterial growth and polysaccharide production of Streptococcus pneumoniae serotype 14 was studied. The presence in the supernatant of serotype-specific CPS (capsular polysaccharide) during growth was followed by size-exclusion HPLC and, in parallel, confirmed by using a specific latex reagent. The agitation level did not affect the production behaviour, whereas pH maintenance above 6 strongly enhanced both growth and CPS production throughout the cultivation period in flasks. Production of high-molecular-mass polysaccharide was found to be maximal between 5 and 6 h of cultivation, at the end of the exponential phase. By laser light scattering, 90% of this purified CPS product showed a Mw (molecular mass) range from 350 to 1500 kDa, with an average Mw of 921 kDa. Extending the culture to 24 h gave rise to a clear shift of the Mw distribution of the polysaccharide to values lower than 100 kDa. These findings may have strong implications for the large-scale manufacture of the polysaccharide and the associated conjugate vaccine.
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Articulos(SEDE CENTRAL)
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Articulos de SEDE CENTRAL
Citación
Massaldi, Hugo Alberto; Bessio, M. Inés; Suárez, Norma; Texeira, Esther; Rossi, Silvina; et al.; Features of bacterial growth and polysaccharide production of Streptococcus pneumoniae serotype 14; Wiley; Biotechnology and Applied Biochemistry; 55; 1; 1-2010; 37-43
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