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dc.contributor.author
Mccarthy, Cristina Beryl
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dc.contributor.author
Santini, Maria Soledad
dc.contributor.author
Pimenta, Paulo F. P.
dc.contributor.author
Diambra, Luis Anibal
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dc.date.available
2015-05-22T19:00:12Z
dc.date.issued
2013-03-12
dc.identifier.citation
Mccarthy; Christina B. ; Santini, Maria Soledad; Pimenta, Paulo F. P. ; Diambra, Luis Anibal; First Comparative Transcriptomic Analysis of Wild Adult Male and Female Lutzomyia longipalpis, Vector of Visceral Leishmaniasis; Public Library Science; Plos One; 8; 3; 12-3-2013; 58645-58665;
dc.identifier.issn
1932-6203
dc.identifier.uri
http://hdl.handle.net/11336/525
dc.description.abstract
Leishmaniasis is a vector-borne disease with a complex epidemiology and ecology. Visceral leishmaniasis (VL) is its most severe clinical form as it results in death if not treated. In Latin America VL is caused by the protist parasite Leishmania infantum (syn. chagasi) and transmitted by Lutzomyia longipalpis. This phlebotomine sand fly is only found in the New World, from Mexico to Argentina. However, due to deforestation, migration and urbanisation, among others, VL in Latin America is undergoing an evident geographic expansion as well as dramatic changes in its transmission patterns. In this context, the first VL outbreak was recently reported in Argentina, which has already caused 7 deaths and 83 reported cases. Insect vector transcriptomic analyses enable the identification of molecules involved in the insect?s biology and vector-parasite interaction. Previous studies on laboratory reared Lu. longipalpis have provided a descriptive repertoire of gene expression in the whole insect, midgut, salivary gland and male reproductive organs. Nevertheless, the study of wild specimens would contribute a unique insight into the development of novel bioinsecticides. Given the recent VL outbreak in Argentina and the compelling need to develop appropriate control strategies, this study focused on wild male and female Lu. longipalpis from an Argentine endemic (Posadas, Misiones) and a Brazilian non-endemic (Lapinha Cave, Minas Gerais) VL location. In this study, total RNA was extracted from the sand flies, submitted to sequence independent amplification and high-throughput pyrosequencing. This is the first time an unbiased and comprehensive transcriptomic approach has been used to analyse an infectious disease vector in its natural environment. Transcripts identified in the sand flies showed characteristic profiles which correlated with the environment of origin and with taxa previously identified in these same specimens. Among these, various genes represented putative targets for vector control via RNA interference (RNAi).
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Public Library Science
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Transcriptomics
dc.subject
Leishmaniasis
dc.subject
Lutzomyia Longipalpis
dc.subject
Bioinformatics
dc.subject.classification
Ciencias Naturales y Exactas
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
Biología Celular, Microbiología
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dc.title
First Comparative Transcriptomic Analysis of Wild Adult Male and Female Lutzomyia longipalpis, Vector of Visceral Leishmaniasis
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.journal.volume
8
dc.journal.number
3
dc.journal.pagination
58645-58665
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
San Francisco
dc.description.fil
Fil: Mccarthy; Christina B.. Universidad Nacional de la Plata. Centro Regional de Estudios Genomicos;
dc.description.fil
Fil: Santini, Maria Soledad. Dir.nacional de Instituto de Investigacion. Adm.nacional de Laboratorio E Instituto de Salud "dr.c.g.malbran". Centro Nacional de Diagnos.e Inv.en Endemo-epidemias;
dc.description.fil
Fil: Pimenta, Paulo F. P.. LABORATORY OF MEDICAL ENTOMOLOGY, CENTRO DE PESQUISAS R;
dc.description.fil
Fil: Diambra, Luis Anibal. Universidad Nacional de la Plata. Centro Regional de Estudios Genomicos;
dc.journal.title
Plos One
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/doi:10.1371/journal.pone.0058645
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