Artículo
CREB3L1-mediated functional and structural adaptation of the secretory pathway in hormone-stimulated thyroid cells
García, Iris Alejandra
; Torres Demichelis, Vanina Andrea
; Viale, Diego Luis
; Di Giusto, Pablo
; Ezhova, Yulia; Polishchuk, Roman S.; Sampieri, Luciana
; Martinez, Hernán; Sztul, Elizabeth; Alvarez, Cecilia Ines
Fecha de publicación:
15/12/2017
Editorial:
Company of Biologists
Revista:
Journal of Cell Science
ISSN:
0021-9533
e-ISSN:
1477-9137
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Many secretory cells increase the synthesis and secretion of cargo proteins in response to specific stimuli. How cells couple increased cargo load with a coordinate rise in secretory capacity to ensure efficient transport is not well understood. We used thyroid cells stimulated with thyrotropin (TSH) to demonstrate a coordinate increase in the production of thyroid-specific cargo proteins and ER-Golgi transport factors, and a parallel expansion of the Golgi complex. TSH also increased expression of the CREB3L1 transcription factor, which alone caused amplified transport factor levels and Golgi enlargement. Furthermore, CREB3L1 potentiated the TSH-induced increase in Golgi volume. A dominant-negative CREB3L1 construct hampered the ability of TSH to induce Golgi expansion, implying that this transcription factor contributes to Golgi expansion. Our findings support a model in which CREB3L1 acts as a downstream effector of TSH to regulate the expression of cargo proteins, and simultaneously increases the synthesis of transport factors and the expansion of the Golgi to synchronize the rise in cargo load with the amplified capacity of the secretory pathway.
Palabras clave:
Creb3l1
,
Frtl-5
,
Golgi
,
Membrane Traffic
,
Secretory Pathway
,
Tsh
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Colecciones
Articulos(CIBICI)
Articulos de CENTRO DE INV.EN BIOQUI.CLINICA E INMUNOLOGIA
Articulos de CENTRO DE INV.EN BIOQUI.CLINICA E INMUNOLOGIA
Citación
García, Iris Alejandra; Torres Demichelis, Vanina Andrea; Viale, Diego Luis; Di Giusto, Pablo; Ezhova, Yulia; et al.; CREB3L1-mediated functional and structural adaptation of the secretory pathway in hormone-stimulated thyroid cells; Company of Biologists; Journal of Cell Science; 130; 24; 15-12-2017; 4155-4167
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