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dc.contributor.author
Cruz Gaitán, Ana María
dc.contributor.author
Torres Ruíz, Nadia Magali
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Carri, Nestor Gabriel
dc.date.available
2018-06-26T16:16:42Z
dc.date.issued
2015-06
dc.identifier.citation
Cruz Gaitán, Ana María; Torres Ruíz, Nadia Magali; Carri, Nestor Gabriel; Embryonic neural stem cells in a 3D bioassay for trophic stimulation studies; Pergamon-Elsevier Science Ltd; Brain Research Bulletin; 115; 6-2015; 37-44
dc.identifier.issn
0361-9230
dc.identifier.uri
http://hdl.handle.net/11336/50080
dc.description.abstract
Progenitors were discovered in the corpus striatum several years ago, but little is known about their proliferation and differentiation. The aim of this study was to analyze embryonic progenitor cells from the corpus striatum using a bioassay with trophic stimulation. Primary cells obtained from brains of rat embryos at E13-14 were dissected from striatum niches and cultured in stem cell media. These floating dispersed cells clumped together to forming floating bodies like irregular spheres (spheroids), which were placed in type I collagen gel and cultured under basal conditions or with the addition of NGF, NT-3, or NTN. Optimum growth of neurites was obtained, and after 24 and 48. h, they were measured for number and length. The expression of proliferation markers such as PCNA and Ki67, and of neural progenitor markers such as GFAP, nestin, vimentin, O4, A2B5, Pax6, S100, TubIII, and NeuN, was then analyzed. The initial behavior in cell cultures showed distinguishable spheroids that, when placed in 3D gels and with trophic support, generated neurites. A similar effect was observed in glial cell outgrowth from the spheroids. Our assay showed high reproducibility, short culture time, and high resolution for tracing neuron-neurite outgrowth or visualizing glial outgrowth in a few hours.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Pergamon-Elsevier Science Ltd
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Collagen
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Neural Progenitors
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Neural Spheroid
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Ngf
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Nt-3
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Ntn
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Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Embryonic neural stem cells in a 3D bioassay for trophic stimulation studies
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-06-26T13:46:17Z
dc.journal.volume
115
dc.journal.pagination
37-44
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: Cruz Gaitán, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina
dc.description.fil
Fil: Torres Ruíz, Nadia Magali. Centro de Investigación TopHealth; México
dc.description.fil
Fil: Carri, Nestor Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina
dc.journal.title
Brain Research Bulletin
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0361923015000672
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.brainresbull.2015.04.006
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