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dc.contributor.author
Fratebianchi de la Parra, Dante  
dc.contributor.author
Acosta, Marina Alejandra  
dc.contributor.author
Cavalitto, Sebastian Fernando  
dc.date.available
2018-06-14T13:59:35Z  
dc.date.issued
2018-03  
dc.identifier.citation
Fratebianchi de la Parra, Dante; Acosta, Marina Alejandra; Cavalitto, Sebastian Fernando; Harnessing soybean hulls for improved polygalacturonase production by Aspergillus sojae through fine-tuning of ambient pH; John Wiley & Sons Ltd; Journal of Chemical Technology and Biotechnology; 93; 3; 3-2018; 667-674  
dc.identifier.issn
0268-2575  
dc.identifier.uri
http://hdl.handle.net/11336/48609  
dc.description.abstract
BACKGROUND: Soybean hulls result from the processing of the bean for producing oil and protein products. This by-product generated massively in America has virtually no commercial value, so substantial effort is being applied to its exploitation for generating value-added goods. This work evaluates soybean hulls as inducer of the production of pectinolytic enzymes, through optimization studies regarding polygalacturonase production by Aspergillus sojae in submerged cultures. RESULTS: A 2-fold improvement in polygalacturonase yield was found by varying the initial pH of the culture in a very narrow acid pH range (2.40–2.80). The optimized fermentation process was successfully transferred to stirred-tank bioreactors in terms of volumetric productivity, and final polygalacturonase yields were 42 U mL-1 and 1.39 U g-1 soybean hulls, which are among the highest reported with this by-product. Morphological characterization of A. sojae during cultivation showed that the fungus mainly developed in dispersed mycelia at initial pH of 2.40–2.80 while, conversely, fungal pellets predominated in cultures performed at initial pH of 5.40. CONCLUSION: High enzyme titers are possibly connected to the formation of dispersed mycelia, as well as to acid-induced expression of the respective gene/s. It is foreseen that this data will be helpful regarding the production of fungal pectinases or other acid-induced enzymes. © 2017 Society of Chemical Industry.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
John Wiley & Sons Ltd  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Aspergillus Sojae  
dc.subject
Initial Culture Ph  
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Polygalacturonase  
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Soybean Hull  
dc.subject.classification
Biotecnología Industrial  
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Biotecnología Industrial  
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INGENIERÍAS Y TECNOLOGÍAS  
dc.title
Harnessing soybean hulls for improved polygalacturonase production by Aspergillus sojae through fine-tuning of ambient pH  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-06-13T14:20:26Z  
dc.journal.volume
93  
dc.journal.number
3  
dc.journal.pagination
667-674  
dc.journal.pais
Reino Unido  
dc.journal.ciudad
Londres  
dc.description.fil
Fil: Fratebianchi de la Parra, Dante. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina  
dc.description.fil
Fil: Acosta, Marina Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina  
dc.description.fil
Fil: Cavalitto, Sebastian Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina  
dc.journal.title
Journal of Chemical Technology and Biotechnology  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1002/jctb.5408  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jctb.5408