Artículo
TRPC3 participates in angiotensin II type 1 receptor-dependent stress-induced slow increase in intracellular Ca2+ concentration in mouse cardiomyocytes
Yamaguchi, Yohei; Iribe, Gentaro; Kaneko, Toshiyuki; Takahashi, Ken; Numaga-Tomita, Takuro; Nishida, Motohiro; Birnbaumer, Lutz
; Naruse, Keiji
Fecha de publicación:
01/2017
Editorial:
Springer Tokyo
Revista:
Journal Of Physiological Sciences
ISSN:
1880-6546
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
When a cardiac muscle is held in a stretched position, its [Ca2+] transient increases slowly over several minutes in a process known as stress-induced slow increase in intracellular Ca2+ concentration ([Ca2+]i) (SSC). Transient receptor potential canonical (TRPC) 3 forms a non-selective cation channel regulated by the angiotensin II type 1 receptor (AT1R). In this study, we investigated the role of TRPC3 in the SSC. Isolated mouse ventricular myocytes were electrically stimulated and subjected to sustained stretch. An AT1R blocker, a phospholipase C inhibitor, and a TRPC3 inhibitor suppressed the SSC. These inhibitors also abolished the observed SSC-like slow increase in [Ca2+]i induced by angiotensin II, instead of stretch. Furthermore, the SSC was not observed in TRPC3 knockout mice. Simulation and immunohistochemical studies suggest that sarcolemmal TRPC3 is responsible for the SSC. These results indicate that sarcolemmal TRPC3, regulated by AT1R, causes the SSC.
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Articulos(OCA HOUSSAY)
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Citación
Yamaguchi, Yohei; Iribe, Gentaro; Kaneko, Toshiyuki; Takahashi, Ken; Numaga-Tomita, Takuro; et al.; TRPC3 participates in angiotensin II type 1 receptor-dependent stress-induced slow increase in intracellular Ca2+ concentration in mouse cardiomyocytes; Springer Tokyo; Journal Of Physiological Sciences; 68; 2; 1-2017; 153-164
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