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dc.contributor.author
Sanz Fernández, María  
dc.contributor.author
Rodríguez Serrano, María  
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Sevilla Perea, Ana  
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Pena, Liliana Beatriz  
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Mingorance, M. Dolores  
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Sandalio, Luisa M.  
dc.contributor.author
Romero Puertas, María C.  
dc.date.available
2018-06-05T14:09:15Z  
dc.date.issued
2017-08  
dc.identifier.citation
Sanz Fernández, María; Rodríguez Serrano, María; Sevilla Perea, Ana; Pena, Liliana Beatriz; Mingorance, M. Dolores; et al.; Screening Arabidopsis mutants in genes useful for phytoremediation; Elsevier Science; Journal of Hazardous Materials; 335; 8-2017; 143-151  
dc.identifier.issn
0304-3894  
dc.identifier.uri
http://hdl.handle.net/11336/47291  
dc.description.abstract
Emissions of heavy metals have risen over the past 200 years and significantly exceed those from natural sources. Phytoremediation strategies may be able to recover soil productivity in self-sustaining ecosystems; however, our knowledge of the molecular mechanisms involved in plant heavy-metal perception and signalling is scarce. The aim of this study was to assemble a ?molecular tool box? of genes useful for phytoremediation. To identify mutants with different heavy-metal-tolerance, we first selected a medium from mixtures containing three metals based on their presence in two Spanish mining areas and then screened about 7000 lines of Arabidopsis T-DNA mutants and found 74 lines more resistant and 56 more susceptible than the wild type (WT). Classification of the genes showed that they were mainly linked to transport, protein modification and signalling, with RNA metabolism being the most representative category in the resistant phenotypes and protein metabolism in the sensitive ones. We have characterized one resistant mutant, Athpp9 and one sensitive, Atala4. These mutants showed differences in growth and metal translocation. Additionally, we found that these mutants keep their phenotype in amended former soils, suggesting that these genes may be useful for phytoremediation and the recovery of contaminated soils.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier Science  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Arabidopsis  
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Atpase  
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Copper  
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Cadmium  
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Chaperons  
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Chrome  
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Heavy Metals  
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Mining  
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Oxidative Stress  
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Screening  
dc.subject.classification
Otras Ciencias Biológicas  
dc.subject.classification
Ciencias Biológicas  
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS  
dc.title
Screening Arabidopsis mutants in genes useful for phytoremediation  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-06-04T17:01:07Z  
dc.journal.volume
335  
dc.journal.pagination
143-151  
dc.journal.pais
Países Bajos  
dc.journal.ciudad
Amsterdam  
dc.description.fil
Fil: Sanz Fernández, María. Consejo Superior de Investigaciones Científicas; España  
dc.description.fil
Fil: Rodríguez Serrano, María. Consejo Superior de Investigaciones Científicas; España  
dc.description.fil
Fil: Sevilla Perea, Ana. IACT; España  
dc.description.fil
Fil: Pena, Liliana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina  
dc.description.fil
Fil: Mingorance, M. Dolores. IACT; España  
dc.description.fil
Fil: Sandalio, Luisa M.. Consejo Superior de Investigaciones Científicas; España  
dc.description.fil
Fil: Romero Puertas, María C.. Consejo Superior de Investigaciones Científicas; España  
dc.journal.title
Journal of Hazardous Materials  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1016/j.jhazmat.2017.04.021  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0304389417302637