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dc.contributor.author
Vila, Silvia Karina
dc.contributor.author
González, Ana María
dc.contributor.author
Rey de Badaró, Hebe Yolanda
dc.contributor.author
Mroginski, Luis Amado
dc.date.available
2018-05-31T12:16:50Z
dc.date.issued
2009-07
dc.identifier.citation
Vila, Silvia Karina; González, Ana María; Rey de Badaró, Hebe Yolanda; Mroginski, Luis Amado; Somatic embryogenesis and plant regeneration in Cedrela fissilis; Springer; Biologia Plantarum; 53; 2; 7-2009; 383-386
dc.identifier.issn
0006-3134
dc.identifier.uri
http://hdl.handle.net/11336/46754
dc.description.abstract
Somatic embryos were obtained from immature zygotic embryos of Cedrela fissilis Well. (Meliaceae), after a culture period of 12 months, with regular subcultures every 6 - 8 weeks. Callus was developed on explants in 2 months on Murashige and Skoog (MS) medium containing 2,4 dichlorophenoxyacetic acid (2,4-D) or picloram (PIC). When the calli were transferred to fresh medium, embryogenic tissue appeared on MS + 45 µM 2,4-D, or 22.5 µM 2,4-D + 0.4 µM 6-benzyladenine (BA), or 20.7 µM PIC after 6 months. Sub-culture of embryogenic tissue in MS medium supplemented with 4.5 µM 2,4-D resulted in the differentiation into somatic embryos after further 4 months. Repeated secondary somatic embryogenesis was achieved by regular subculture on this medium. Maturation and conversion of somatic embryos into plantlets was achieved on MS medium without plant growth regulators and the conversion frequency was approximately 12.5 %. The plantlets were successfully acclimatized in pots with soil. Histological studies showed that somatic embryos had no detectable connection with the mother explants and that somatic embryos in advanced stages were bipolar with shoot and root apical meristems, they contained vascular system and showed typical characteristics of a somatic dicotyledonous embryo.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Cedela Fissilis
dc.subject
Callus Induction
dc.subject
Growth Regulators
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Secondary Embryogenesis
dc.subject.classification
Ciencias de las Plantas, Botánica
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Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Somatic embryogenesis and plant regeneration in Cedrela fissilis
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-05-21T16:57:29Z
dc.identifier.eissn
1573-8264
dc.journal.volume
53
dc.journal.number
2
dc.journal.pagination
383-386
dc.journal.pais
Alemania
dc.description.fil
Fil: Vila, Silvia Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
dc.description.fil
Fil: González, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
dc.description.fil
Fil: Rey de Badaró, Hebe Yolanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
dc.description.fil
Fil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
dc.journal.title
Biologia Plantarum
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s10535-009-0072-5
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s10535-009-0072-5
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