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dc.contributor.author
Montanari, Sonia
dc.contributor.author
Dayan, Victor
dc.contributor.author
Yannarelli, Gustavo Gabriel
dc.contributor.author
Billia, Filio
dc.contributor.author
Viswanathan, Sowmya
dc.contributor.author
Connelly, Kim A.
dc.contributor.author
Keating, Armand
dc.date.available
2018-04-17T17:08:11Z
dc.date.issued
2015-11
dc.identifier.citation
Montanari, Sonia; Dayan, Victor; Yannarelli, Gustavo Gabriel; Billia, Filio; Viswanathan, Sowmya; et al.; Mesenchymal stromal cells improve cardiac function and left ventricular remodeling in a heart transplantation model; Elsevier Science Inc; Journal of Heart and Lung Transplantation; 34; 11; 11-2015; 1481-1488
dc.identifier.issn
1053-2498
dc.identifier.uri
http://hdl.handle.net/11336/42282
dc.description.abstract
Background: Ischemia/reperfusion (I/R) injury is an inevitable consequence of organ transplantation and a major determinant of patient and graft survival in heart transplantation. Bone marrow–mesenchymal stromal cell (BM-MSC) treatment is a potentially effective cell therapy for cardiac disease. We investigated the effects of intravenous delivery of BM-MSCs in the acute phase post-transplant in a heterotopic heart transplantation (HHT) model associated with I/R injury. Methods: Hearts of wild-type Lewis (WT LEW) rats were harvested and transplanted heterotopically into the necks of recipient WT LEW rats. Forty-eight hours after HHT, BM-MSCs were injected intravenously into animals in the experimental group, whereas controls received normal saline (NS). Results: Eight days after BM-MSC injection, fractional shortening of transplanted hearts was significantly higher and left ventricular systolic diameter was lower in the BM-MSC group compared with controls, whereas no differences were found 28 days after infusion. A reduction in ventricular remodeling and cardiac fibrosis was observed by histochemical analysis and confirmed by cardiac magnetic resonance imaging in the BM-MSC group. The perivascular stromal cells’ density and the number of capillaries were increased whereas the number of apoptotic cells decreased significantly in transplanted hearts in the BM-MSC group compared with the NS group. Conclusions: We showed early improvement in cardiac function and subsequent enhanced ventricular remodeling, reduced cardiac fibrosis, augmented neo-vascularization and decreased cardiomyocyte apoptosis of the transplanted heart in a heterotopic transplantation model after intravenous infusion of BM-derived MSCs. Our data suggest that clinical studies with BM-MSCs are warranted to understand their effects on cardiac graft and transplant recipient survival.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science Inc
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Mesenchymal Stromal Cells
dc.subject
Heart Transplantation
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Remodeling
dc.subject.classification
Otras Biotecnologías de la Salud
dc.subject.classification
Biotecnología de la Salud
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Mesenchymal stromal cells improve cardiac function and left ventricular remodeling in a heart transplantation model
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-04-17T13:49:02Z
dc.journal.volume
34
dc.journal.number
11
dc.journal.pagination
1481-1488
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: Montanari, Sonia. University Health Network; Canadá. University of Toronto; Canadá
dc.description.fil
Fil: Dayan, Victor. University Health Network; Canadá
dc.description.fil
Fil: Yannarelli, Gustavo Gabriel. University Health Network; Canadá. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Billia, Filio. Toronto General Hospital; Canadá
dc.description.fil
Fil: Viswanathan, Sowmya. University Health Network; Canadá
dc.description.fil
Fil: Connelly, Kim A.. Keenan Research Centre; Canadá
dc.description.fil
Fil: Keating, Armand. University Health Network; Canadá. University of Toronto; Canadá
dc.journal.title
Journal of Heart and Lung Transplantation
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.healun.2015.05.008
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1053249815012796
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