Artículo
Novel linear megaplasmid from Brevibacterium sp. isolated from extreme environment
Dib, Julian Rafael
; Wagenknecht, Martin; Hill, Russell T.; Farias, Maria Eugenia
; Meinhardt, Friedhelm
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Fecha de publicación:
05/2010
Editorial:
Wiley VCH Verlag
Revista:
Journal of Basic Microbiology
ISSN:
0233-111X
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Brevibacterium sp. Ap13, isolated from flamingo's feces in Laguna Aparejos, a high-altitude lake located at approximately 4,200 m in the northwest of Argentina was previously found to be resistant to multiple antibiotics, and was therefore screened for plasmids that may be implicated in antibiotic resistance. Brevibacterium sp. Ap13 was found to contain two plasmids of approximately 87 and 436 kb, designated pAP13 and pAP13c, respectively. Only pAP13 was stably maintained and was extensively characterized by pulsed-field gel electrophoresis to reveal that this plasmid is linear and likely has covalently linked terminal proteins associated with its 5' ends. This is the first report of a linear plasmid in the genus Brevibacterium and may provide a new tool for genetic manipulation of this commercially important genus.
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Licencia
Identificadores
Colecciones
Articulos(PROIMI)
Articulos de PLANTA PILOTO DE PROC.IND.MICROBIOLOGICOS (I)
Articulos de PLANTA PILOTO DE PROC.IND.MICROBIOLOGICOS (I)
Citación
Dib, Julian Rafael; Wagenknecht, Martin; Hill, Russell T.; Farias, Maria Eugenia; Meinhardt, Friedhelm; Novel linear megaplasmid from Brevibacterium sp. isolated from extreme environment; Wiley VCH Verlag; Journal of Basic Microbiology; 50; 3; 5-2010; 280-284
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