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dc.contributor.author
Marconi, Patricia Laura
dc.contributor.author
Alvarez, Maria Alejandra
dc.contributor.author
Klykov. S. P.
dc.contributor.author
Kurakov, V. V.
dc.date.available
2016-02-10T18:59:17Z
dc.date.issued
2014-01
dc.identifier.citation
Marconi, Patricia Laura; Alvarez, Maria Alejandra; Klykov. S. P.; Kurakov, V. V.; Application of a Mathematical Model for Production of Recombinant Antibody 14D9 by Nicotiana tabacum Cell Suspension Batch Culture; Bioprocess International; Bioprocess; 12; 1; 1-2014; 42-49
dc.identifier.issn
1542-6319
dc.identifier.uri
http://hdl.handle.net/11336/4108
dc.description.abstract
One of the bottlenecks in biopharming is the scalability and modeling of batch cultures at bench scale, largely because bioreactors and mathematical models were originally designed for microbe cultures. Thus, a mathematical model that acknowledges kinetic and growth processes, basic aspect of cell structure, and physiology and productivity is needed. We proposed to apply a new combination of structured and unstructured mathematical models for plant cell cultivation in a bioreactor.The mathematical model is based on the theory of energy-limited growth that allows precise evaluation of a cell population age structure into the bioreactor. We have scaledthe full antibody r14D9 expressed in N. tabacumcell suspension from a 225 mL-Erlenmeyer flask to 2L-bioreactor and applied the above mentioned mathematical model in order to estimate and predict the growth rate of cell cultures and the accumulation of the recombinant protein. Under the proposed model two inoculum sizes were used in order to obtain experimental data, specific growth rates and productivity, which allow the analysis of the predictions under different conditions.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Bioprocess International
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Catalytic Antibodies
dc.subject
Molecular Farming
dc.subject
Plant Bioreactor
dc.subject
Mathematical Model
dc.subject.classification
Bioprocesamiento Tecnológico, Biocatálisis, Fermentación
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Biotecnología Industrial
dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
dc.title
Application of a Mathematical Model for Production of Recombinant Antibody 14D9 by Nicotiana tabacum Cell Suspension Batch Culture
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.journal.volume
12
dc.journal.number
1
dc.journal.pagination
42-49
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Eugene
dc.description.fil
Fil: Marconi, Patricia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina
dc.description.fil
Fil: Alvarez, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina
dc.description.fil
Fil: Klykov. S. P.. PHARMAREGION; Rusia
dc.description.fil
Fil: Kurakov, V. V.. PHARMAREGION; Rusia
dc.journal.title
Bioprocess
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.bioprocessintl.com/upstream-processing/assays/mathematical-model-for-production-of-recombinant-antibody-14d9-by-nicotiana-tabacum-cell-suspension-batch-culture-349252/
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