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dc.contributor.author
Vidoz, María Laura
dc.contributor.author
Klusacek, Pablo Antonio
dc.contributor.author
Rey de Badaró, Hebe Yolanda
dc.contributor.author
Mroginski, Luis Amado
dc.date.available
2018-04-05T20:42:21Z
dc.date.issued
2006-12
dc.identifier.citation
Vidoz, María Laura; Klusacek, Pablo Antonio; Rey de Badaró, Hebe Yolanda; Mroginski, Luis Amado; In vitro plant regeneration of Arachis correntina (Leguminosae) through somatic embryogenesis and organogenesis; Springer; Plant Cell, Tissue and Organ Culture; 86; 1; 12-2006; 111-115
dc.identifier.issn
0167-6857
dc.identifier.uri
http://hdl.handle.net/11336/41004
dc.description.abstract
In vitro protocols for plant regeneration of Arachis correntina through both somatic embryogenesis and organogenesis were developed using immature leaves as explants. Morphologically normal somatic embryos were obtained on culture media composed of 20.70 or 41.41 μM picloram (PIC) with the addition of 0.044 μM 6-benzylaminopurine (BA), resulting in a 33 and 24% of conversion into plants, respectively. The source of explants and the developmental stage of the leaves had a marked effect on somatic embryogenesis. The second folded immature leaves from in vitro growing plants were the most responsive producing up to 30% embryogenesis in MS+41.41 μM PIC. Embryos converted into plants after transfer to MS medium devoid of growth regulators and these plants were successfully acclimatised. Adventitious shoots were obtained on culture media supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) or naphthaleneacetic acid (NAA) with or without 0.044 μM BA, achieving plant regeneration in the induction media. The highest percentage of bud formation was obtained on culture medium composed of␣MS+10.74 μM NAA+0.044 μM BA (12.5%). Roots were formed on all culture media tested. Regenerated plants were transferred to pots and grew well under greenhouse conditions
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Arachis Correntina
dc.subject
Explant Type
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Organogenesis
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Plant Regeneration
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Somatic Embryogenesis
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Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
In vitro plant regeneration of Arachis correntina (Leguminosae) through somatic embryogenesis and organogenesis
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-03-21T15:29:43Z
dc.identifier.eissn
1573-5044
dc.journal.volume
86
dc.journal.number
1
dc.journal.pagination
111-115
dc.journal.pais
Países Bajos
dc.journal.ciudad
Dordrecht
dc.description.fil
Fil: Vidoz, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
dc.description.fil
Fil: Klusacek, Pablo Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
dc.description.fil
Fil: Rey de Badaró, Hebe Yolanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
dc.description.fil
Fil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
dc.journal.title
Plant Cell, Tissue and Organ Culture
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s11240-005-9062-2
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s11240-005-9062-2
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