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dc.contributor.author
Palacios, Carlos Adolfo
dc.contributor.author
Claus, Juan Daniel
dc.contributor.author
Mattion, Nora Marta
dc.date.available
2016-02-05T12:35:48Z
dc.date.issued
2015-02
dc.identifier.citation
Palacios, Carlos Adolfo; Claus, Juan Daniel; Mattion, Nora Marta; Rotavirus VP6 protein expressed in cell culture by HSV-1-based vectors; Asociación Argentina de Microbiología; Revista Argentina de Microbiología; 47; 1; 2-2015; 80-81
dc.identifier.issn
0325-7541
dc.identifier.uri
http://hdl.handle.net/11336/4043
dc.description.abstract
HSV-1 vectors were developed for the expression of several rotavirus genes. Genes from other pathogens were also expressed, thus generating a set of tools to be tested as new vaccine candidates against In this section, we show two different HSV-1 vectors that express the VP6 protein from different rotavirus strains. Figure 1 shows a defective recombinant virus expressing simultaneously EGFP and VP6 from murine rotavirus strain EC, In Figure 2, an amplicon vector overexpressing VP6 from a Wa strain of human Rotavirus is shown. In both cases, the VP6 proteins were recognized by a monoclonal antibody and were found extensively expressed forming filaments along the cells, showing a typical intra-cellular pattern, in the absence of other rotavirus proteins. These results show that the recombinant proteins maintain the specific capacity of self-assembly of the native protein and, therefore, are potential vaccine candidates to be explored.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Asociación Argentina de Microbiología
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Herpesvirus Vectors
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Rotavirus
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Vp6
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Recombinant Protein
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Virología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Rotavirus VP6 protein expressed in cell culture by HSV-1-based vectors
dc.title
Expresión en cultivo celular de la proteína VP6 de rotavirus mediante el uso de vectores virales basados en HSV-1
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.identifier.eissn
1851-7617
dc.journal.volume
47
dc.journal.number
1
dc.journal.pagination
80-81
dc.journal.pais
Argentina
dc.journal.ciudad
Buenos Aires
dc.description.fil
Fil: Palacios, Carlos Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina
dc.description.fil
Fil: Claus, Juan Daniel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
dc.description.fil
Fil: Mattion, Nora Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina
dc.journal.title
Revista Argentina de Microbiología
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0325754115000127
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.ram.2014.10.002
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.redalyc.org/articulo.oa?id=213038579016
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/issn/0325-7541
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