Artículo
High-throughput modified QuEChERS method for the determination of the mycotoxin tenuazonic acid in wine grapes
Fecha de publicación:
10/2016
Editorial:
Royal Society of Chemistry
Revista:
RSC Advances
ISSN:
2056-9335
e-ISSN:
2046-2069
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
A reliable, simple, fast, inexpensive and robust sample preparation approach for tenuazonic acid (TA) determination in grapes by liquid chromatography with ultraviolet detection (HPLC-UV) is proposed. The method is based on a modified QuEChERS (quick, easy, cheap, effective, rugged and safe) approach using ethyl acetate as extraction solvent. Its involves extraction of 2.5 g sample (plus 2.5 mL 1% formic acid acidified water) with 5 mL of ethyl acetate; drying/cleaning-up of extract with 0.25 g anhydrous CaCl2 and determination by HPLC-UV. The method was optimized and validated achieving a limit of quantification (LOQ) of 0.05 μg g-1. The overall recoveries were 96%, 82% and 97% for grape samples spiked at 0.05, 0.5 and 5 μg g-1, respectively. The method showed excellent reproducibility with RSDs for the above data ≤8% and Horwitz ratios <0.11. The procedure was applied to evaluate the occurrence of TA in grapes and it was quantified at concentrations ranging between 0.057 and 0.595 μg g-1. The methodological development allows a high throughput routine quality control of grapes, adding a valuable tool for the winemaking industry. The method could be applied in an average laboratory and help to understand possible effects related to Alternaria rotting on the final wines elaborated. As well, this is the first report of TA presence in grapes used for wine elaboration, so the results add new knowledge to a growing research area.
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Articulos(IBAM)
Articulos de INST.DE BIOLOGIA AGRICOLA DE MENDOZA
Articulos de INST.DE BIOLOGIA AGRICOLA DE MENDOZA
Citación
Fontana, Ariel Ramón; Prendes, Luciana Paola; Morata, Vilma Ines; Bottini, Ambrosio Rubén; High-throughput modified QuEChERS method for the determination of the mycotoxin tenuazonic acid in wine grapes; Royal Society of Chemistry; RSC Advances; 6; 98; 10-2016; 95670-95679
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