Mostrar el registro sencillo del ítem
dc.contributor.author
Galatro, Andrea Verónica
dc.contributor.author
Puntarulo, Susana Ángela
dc.date.available
2018-03-15T17:01:47Z
dc.date.issued
2016-04
dc.identifier.citation
Galatro, Andrea Verónica; Puntarulo, Susana Ángela; Measurement of nitric oxide (NO) generation rate by chloroplasts employing electron spin resonance (ESR); Humana Press; Methods in Molecular Biology; 1424; 4-2016; 103-112
dc.identifier.isbn
978-1-4939-3600-7
dc.identifier.issn
1064-3745
dc.identifier.uri
http://hdl.handle.net/11336/38904
dc.description.abstract
Chloroplasts are among the more active organelles involved in free energy transduction in plants (photophosphorylation). Nitric oxide (NO) generation by soybean (Glycine max, var ADM 4800) chloroplasts was measured as an endogenous product assessed by electron paramagnetic resonance (ESR) spin-trapping technique. ESR spectroscopy is a methodology employed to detect species with unpaired electrons (paramagnetic). This technology has been successfully applied to different plant tissues and subcellular compartments to asses both, NO content and generation. The spin trap MGD-Fe2+ is extensively employed to efficiently detect NO. Here, we describe a simple methodology to asses NO generation rate by isolated chloroplasts in the presence of either L -Arginine or nitrite (NO2 −) as substrates, since these compounds are required for enzymatic activities considered as the possible sources of NO generation in plants.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Humana Press
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Chloroplasts
dc.subject
Esr
dc.subject
No Generation
dc.subject
No2 −
dc.subject
Nos Like Activity
dc.subject.classification
Otras Ciencias Biológicas
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Measurement of nitric oxide (NO) generation rate by chloroplasts employing electron spin resonance (ESR)
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-03-15T14:04:02Z
dc.journal.volume
1424
dc.journal.pagination
103-112
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Nueva York
dc.description.fil
Fil: Galatro, Andrea Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina
dc.description.fil
Fil: Puntarulo, Susana Ángela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina
dc.journal.title
Methods in Molecular Biology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/978-1-4939-3600-7_9
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/protocol/10.1007%2F978-1-4939-3600-7_9
Archivos asociados