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dc.contributor.author
Rodríguez, María Daniela  
dc.contributor.author
Barchuk, Mónica Lucrecia  
dc.contributor.author
Fonseca, Maria Isabel  
dc.contributor.author
Zapata, Pedro Dario  
dc.contributor.author
Villalba, Laura  
dc.date.available
2018-03-08T21:52:18Z  
dc.date.issued
2015-11  
dc.identifier.citation
Rodríguez, María Daniela; Barchuk, Mónica Lucrecia; Fonseca, Maria Isabel; Zapata, Pedro Dario; Villalba, Laura; Effect of wood flour as carbon source on cellulases and xylanases production by white-rot-fungi native from Misiones; Council for Innovative Research; Journal of Advances in Biotechnology; 5; 1; 11-2015; 526-533  
dc.identifier.issn
2348-6201  
dc.identifier.uri
http://hdl.handle.net/11336/38353  
dc.description.abstract
There is a need to explore lignocellulosic materials to select an adequate substrate for lignocellulolytic enzyme production. Utilization of some residues provides an opportunity to produce high yields of lignocellulolytic enzymes in a simple medium. The aim of the present work was to study the effect of wood flour as a carbon source on the cellulolytic and xylanolytic secretion of white-rot fungi native from Misiones. Fungi were incubated with 5 g Pinus sp. wood flour/L and 5 g Eucalyptus sp. wood flour/L as carbon sources in a reciprocal shaker at 80 rpm and 29ºC for 15 days. Total cellulase, endo-1,4-β-glucanase, β-glucosidase, cellobiohydrolase and endo-1,4-β-xylanase activities were determined in culture supernatants. Tested fungi showed high endo-1,4-β-xylanase activity between 6 and 12 days. Total cellulase showed the highest activity between 12-15 culture days. The test did not show differences among Pycnoporus sanguineus LBM 014, P. sanguineus BAFC 2126, Irpex lacteus BAFC 1171, Irpex sp. LBM 032, Irpex sp. LBM 034 and Lenzites elegans BAFC 2127, showing the highest activity for this group regard the others strains. I. lacteus BAFC 1171 was the strain with major endo-1,4-β-glucanase activity at day 9 (847 U/L). In the case of β-glucosidase and cellobiohydrolase, P. sanguineus BAFC 2126 was the strain with the highest activity between 12-15 culture days (18 U/L), and between 9-15 culture days (39 U/L), respectively. Wood flour proved to be a suitable carbon source to produce hydrolytic enzymes. I. lacteus BAFC 1171 and P. sanguineus BAFC 2126 have potential for cellulase production whereas P. sanguineus LBM 008 is a good endo-1,4-β-xylanase producer.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Council for Innovative Research  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Cellulase  
dc.subject
Endo-1,4-B-Xylanase  
dc.subject
White-Rot Fungi  
dc.subject
Wood Flour  
dc.subject.classification
Biotecnología Industrial  
dc.subject.classification
Biotecnología Industrial  
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INGENIERÍAS Y TECNOLOGÍAS  
dc.title
Effect of wood flour as carbon source on cellulases and xylanases production by white-rot-fungi native from Misiones  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-03-01T13:51:08Z  
dc.identifier.eissn
2348-6201  
dc.journal.volume
5  
dc.journal.number
1  
dc.journal.pagination
526-533  
dc.journal.pais
India  
dc.journal.ciudad
Punjab  
dc.description.fil
Fil: Rodríguez, María Daniela. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Barchuk, Mónica Lucrecia. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; Argentina. Comision Nacional de Investigacion Cientifica y Tecnologica; Chile  
dc.description.fil
Fil: Fonseca, Maria Isabel. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Zapata, Pedro Dario. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Villalba, Laura. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; Argentina  
dc.journal.title
Journal of Advances in Biotechnology  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://cirworld.com/index.php/jbt/article/view/4855