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dc.contributor.author
Farias, Marta Elena
dc.contributor.author
Manca, Maria Cristina
dc.date.available
2018-03-08T16:45:02Z
dc.date.issued
2000-04
dc.identifier.citation
Farias, Marta Elena; Manca, Maria Cristina; Purification and partial characterization of Oenococcus oeni exoprotease; Wiley Blackwell Publishing, Inc; FEMS Microbiology Letters; 185; 2; 4-2000; 263-266
dc.identifier.issn
0378-1097
dc.identifier.uri
http://hdl.handle.net/11336/38277
dc.description.abstract
The exoprotease from Oenococcus oeni produced in stress conditions was purified to homogeneity in two steps, a 14-fold increase of specific activity and a 44% recovery of proteinase activity. The molecular mass was estimated to be 33.1 kDa by gel filtration and 17 kDa by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE). These results suggest that the enzyme is a dimer consisting of two identical subunits. Optimal conditions for activity on grape juice were 25°C and a pH of 4.5. Incubation at 70°C, 15 min, destroyed proteolytic activity. The SDS–PAGE profile shows that the enzyme was able to degrade the grape juice proteins at a significantly high rate. The activity at low pH and pepstatin A inhibition indicate that this enzyme is an aspartic protease. The protease activity increases at acidic pH suggesting that it could be involved in the wine elaboration.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject.classification
Otras Ciencias Biológicas
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Purification and partial characterization of Oenococcus oeni exoprotease
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-03-06T17:03:08Z
dc.journal.volume
185
dc.journal.number
2
dc.journal.pagination
263-266
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Farias, Marta Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
dc.description.fil
Fil: Manca, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
dc.journal.title
FEMS Microbiology Letters
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/j.1574-6968.2000.tb09072.x
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.2000.tb09072.x/abstract
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