Artículo
Purification and characterization of invertase from Lactobacillus reuteri CRL 1100
Fecha de publicación:
12/2000
Editorial:
Springer
Revista:
Current Microbiology
ISSN:
0343-8651
e-ISSN:
1432-0991
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The invertase of Lactobacillus reuteri CRL 1100 is a glycoprotein composed by a single subunit with a molecular weight of 58 kDa. The enzyme was stable below 45°C over a wide pH range (4.5-7.0) with maximum activity at pH 6.0 and 37°C. The invertase activity was significantly inhibited by bivalent metal ions (Ca++, Cu++, Cd++, and Hg++), β-mercaptoethanol, and dithiothreitol and partially improved by ethylenediaminetetraacetic acid. The enzyme was purified 32 times over the crude extract by gel filtration and ion-exchange chromatography with a recovery of 17%. The K(m) and V(max) values for sucrose were 6.66 mm and 0.028 μmol/min, respectively. An invertase is purified and characterized for the first time in Lactobacillus, and it proved to be a β-fructofuranosidase.
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CERELA)
Articulos de CENTRO DE REFERENCIA PARA LACTOBACILOS (I)
Articulos de CENTRO DE REFERENCIA PARA LACTOBACILOS (I)
Citación
De Ginés, Silvia Cuezzo; Maldonado, María C.; Font, Graciela Maria; Purification and characterization of invertase from Lactobacillus reuteri CRL 1100; Springer; Current Microbiology; 40; 3; 12-2000; 181-184
Compartir
Altmétricas