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dc.contributor.author Pasteris, Sergio Enrique
dc.contributor.author Montel Mendoza, María Gabriela
dc.contributor.author Llanos, Ricardo J.
dc.contributor.author Pucci Alcaide, Franco J.
dc.contributor.author Nader, Maria Elena Fatima
dc.date.available 2018-02-19T17:53:23Z
dc.date.issued 2017-05
dc.identifier.citation Pasteris, Sergio Enrique; Montel Mendoza, María Gabriela; Llanos, Ricardo J.; Pucci Alcaide, Franco J.; Nader, Maria Elena Fatima; Preliminary assessment of in vivo safety of potentially probiotic lactic acid bacteria for American bullfrog culture; Wiley Blackwell Publishing, Inc; Aquaculture Research; 48; 5; 5-2017; 2157-2172
dc.identifier.issn 1355-557X
dc.identifier.uri http://hdl.handle.net/11336/36723
dc.description.abstract The effect of the administration of beneficial lactic acid bacteria (LAB): Lactococcus lactis CRL 1584, L. lactis CRL 1827, Enterococcus gallinarum CRL 1826 and combined CRL 1584+ CRL 1826 on the development of bullfrog embryos from the hatching stage until 31 days (tadpole) was evaluated. In vitro assays indicated that 103, 106 and 109 CFU mL−1 single LAB strains remained viable until 24 h in 10% Ringer. Around 109 CFU mL−1 LAB (individually and combined) were used in an experimental design built to evaluate their effect when administrated at different intervals (three 7-day cycles with 5-day rest periods in between) to embryos until day 31. The highest potentially beneficial population (LAB) numbers were detected in the LAB-treated groups. All the LAB-treatments increased it and were significantly higher than the controls. Although the highest, potentially pathogenic, population (Red-Leg Syndrome-RLS-related pathogens) numbers were detected in the control and the lowest in the CRL 1584+ CRL 1826-treated group, they did not differ significantly. Stereoscopic studies showed no malformations in any LAB-treated group and all the specimens reached the same stage of their biological cycle with a survival >94%. The histological structure of target organs for RLS-associated pathogens (intestine and skin) and stomach was not affected and the spleen was developed. Only the LAB-treated groups showed microorganisms associated with the intestinal mucus, without inflammatory reaction in the lamina propria. This is the first report on the evaluation of the absence of adverse effects after LAB administration to bullfrog embryos using indigenous microorganisms.
dc.format application/pdf
dc.language.iso eng
dc.publisher Wiley Blackwell Publishing, Inc
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject BULLFROG CULTURE
dc.subject LACTIC ACID BACTERIA
dc.subject MORPHOLOGICAL STUDIES
dc.subject PROBIOTICS
dc.subject RANICULTURE
dc.subject.classification Agricultura
dc.subject.classification Agricultura, Silvicultura y Pesca
dc.subject.classification CIENCIAS AGRÍCOLAS
dc.title Preliminary assessment of in vivo safety of potentially probiotic lactic acid bacteria for American bullfrog culture
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-07-13T18:58:52Z
dc.journal.volume 48
dc.journal.number 5
dc.journal.pagination 2157-2172
dc.journal.pais Reino Unido
dc.journal.ciudad Londres
dc.description.fil Fil: Pasteris, Sergio Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
dc.description.fil Fil: Montel Mendoza, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
dc.description.fil Fil: Llanos, Ricardo J.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
dc.description.fil Fil: Pucci Alcaide, Franco J.. Fundación Miguel Lillo; Argentina
dc.description.fil Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
dc.journal.title Aquaculture Research
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/are.13053
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/are.13053


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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)