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dc.contributor.author
Coirini, Hector
dc.contributor.author
Gouezou, M.
dc.contributor.author
Delespierre, B.
dc.contributor.author
Liere, P.
dc.contributor.author
Pianos, A.
dc.contributor.author
Eychenne, B.
dc.contributor.author
Schumacher, Michael
dc.contributor.author
Guennoun, Rachida
dc.date.available
2018-02-08T13:41:46Z
dc.date.issued
2003-12
dc.identifier.citation
Coirini, Hector; Gouezou, M.; Delespierre, B.; Liere, P.; Pianos, A.; et al.; Characterization and regulation of the 3beta-hydroxysteroid dehydrogenase isomerase enzyme in the rat sciatic nerve; Wiley Blackwell Publishing, Inc; Journal of Neurochemistry; 84; 1; 12-2003; 119-126
dc.identifier.issn
0022-3042
dc.identifier.uri
http://hdl.handle.net/11336/36093
dc.description.abstract
In the peripheral nervous system, progesterone (PROG) has a stimulatory effect on myelination. It could be derived from local synthesis, as Schwann cells in culture express the 3beta-hydroxysteroid dehydrogenase (3beta-HSD) and convert pregnenolone (PREG) to PROG. Although 3beta-HSD mRNA can be detected by RT-PCR in peripheral nerves, the activity of the enzyme has so far not been demonstrated and characterized in nerve tissue. In this study, we show that homogenates prepared from rat sciatic nerves contain a functional 3beta-HSD enzyme and we have analysed its kinetic properties and its regulation by steroids. The activity of 3beta-HSD in homogenates was evaluated using 3H-labelled PREG as a substrate and NAD+ as a cofactor, the levels of steroids formed were calculated either by extrapolating the relationship between tritiated peaks obtained by TLC to the initial amount of PREG, or by gas chromatography/mass spectrometry determination. A rapid increase in PROG formation was found between 0 and 50 min of incubation and no further significant changes were observed between 1 and 4 h. The calculated Km value (1.06 +/- 0.19 microm) was close to the values described for the 3beta-HSD type-I and type-IV isoforms. Trilostane, a competitive inhibitor of the 3beta-HSD caused a potent inhibition of the rate of conversion of PREG to PROG (IC50 = 4.06 +/- 2.58 microm). When the effects of different steroids were tested, both oestradiol and PROG significantly inhibited the conversion of PREG to PROG.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Neurosteroids
dc.subject
Pregnenolone
dc.subject
Progesterone
dc.subject
Steroids
dc.subject.classification
Inmunología
dc.subject.classification
Medicina Básica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.subject.classification
Neurociencias
dc.subject.classification
Medicina Básica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Characterization and regulation of the 3beta-hydroxysteroid dehydrogenase isomerase enzyme in the rat sciatic nerve
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-12-28T13:40:35Z
dc.identifier.eissn
1471-4159
dc.journal.volume
84
dc.journal.number
1
dc.journal.pagination
119-126
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Washington DC
dc.description.fil
Fil: Coirini, Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Inserm; Francia
dc.description.fil
Fil: Gouezou, M.. Inserm; Francia
dc.description.fil
Fil: Delespierre, B.. Inserm; Francia
dc.description.fil
Fil: Liere, P.. Inserm; Francia
dc.description.fil
Fil: Pianos, A.. Inserm; Francia
dc.description.fil
Fil: Eychenne, B.. Inserm; Francia
dc.description.fil
Fil: Schumacher, Michael. Inserm; Francia
dc.description.fil
Fil: Guennoun, Rachida. Inserm; Francia
dc.journal.title
Journal of Neurochemistry
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1046/j.1471-4159.2003.01512.x/full
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1046/j.1471-4159.2003.01512.x
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/pmid/12485408
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/12485408
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