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dc.contributor.author
Zambernardi König, María Agustina
dc.contributor.author
Chiodetti, Ana Laura
dc.contributor.author
Meier, Dominik
dc.contributor.author
Cabanne, Ana
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Nachman, Fabio
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Solar, Héctor
dc.contributor.author
Rumbo, Carolina
dc.contributor.author
Gondolesi, Gabriel Eduardo
dc.contributor.author
Rumbo, Martín
dc.date.available
2018-01-15T15:02:42Z
dc.date.issued
2014-09
dc.identifier.citation
Zambernardi König, María Agustina; Cabanne, Ana; Nachman, Fabio; Meier, Dominik; Chiodetti, Ana Laura; Rumbo, Martín; et al.; Immunosuppressive therapies after intestinal transplant modulate the expression of Th1 signature genes during acute cellular rejection. Implications in the search for rejection biomarkers; Wiley Blackwell Publishing, Inc; Clinical Transplantation; 28; 12; 9-2014; 1365-1371
dc.identifier.issn
0902-0063
dc.identifier.uri
http://hdl.handle.net/11336/33220
dc.description.abstract
Background and Aims Acute cellular rejection (ACR) and infections are leading causes of graft loss and death in intestinal transplant patients. Our aim was to evaluate the impact of maintenance immunosuppressive therapies on the expression of pro-inflammatory mediators in small bowel at ACR diagnosis. Materials and Methods We analyzed expression levels of Th1-associated genes, IFNG, CXCL10, and CXCL11 by qPCR in 46 selected graft biopsies unequivocally assigned to mild ACR (n = 14) or normal histopathology and clinical condition (n = 32) from 15 patients receiving two different immunosuppressive (IS) schemes. Double treatment: corticosteroids and tacrolimus (n = 17) and triple treatment: sirolimus or mycophenolate mofetil in addition to the basal therapy (n = 29). Results IFNG, CXCL10, and CXCL11 were induced during rejection (p < 0.05; p < 0.005, and p < 0.05, respectively). However, when rejection and control groups were classified according to immunosuppressive treatment, in the rejection group, significant differences of IFNG, CXCL10, and CXCL11 expression (p < 0.001; p < 0.005, and 0.01, respectively) were detected, whereas no differences were observed in the control group. Conclusion Gene expression of Th1 response mediators is higher during ACR. Triple IS group showed significantly lower expression of pro-inflammatory Th1 mediators during mild ACR indicating that use of these markers to monitor rejection can be affected by the IS treatment used.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Gene Expression
dc.subject
Immunosuppression
dc.subject
Interferon Gamma
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Intestinal Transplant
dc.subject
Rejection
dc.subject.classification
Inmunología
dc.subject.classification
Medicina Básica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Immunosuppressive therapies after intestinal transplant modulate the expression of Th1 signature genes during acute cellular rejection. Implications in the search for rejection biomarkers
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-01-12T16:18:48Z
dc.journal.volume
28
dc.journal.number
12
dc.journal.pagination
1365-1371
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Zambernardi König, María Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Fundación Favaloro; Argentina
dc.description.fil
Fil: Chiodetti, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina
dc.description.fil
Fil: Meier, Dominik. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Favaloro; Argentina
dc.description.fil
Fil: Cabanne, Ana. Fundación Favaloro; Argentina
dc.description.fil
Fil: Nachman, Fabio. Fundación Favaloro; Argentina
dc.description.fil
Fil: Solar, Héctor. Fundación Favaloro; Argentina
dc.description.fil
Fil: Rumbo, Carolina. Fundación Favaloro; Argentina
dc.description.fil
Fil: Gondolesi, Gabriel Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Favaloro; Argentina
dc.description.fil
Fil: Rumbo, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina
dc.journal.title
Clinical Transplantation
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/ctr.12464/abstract
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/ctr.12464
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