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dc.contributor.author
Nikoloff, Noelia
dc.contributor.author
Larramendy, Marcelo Luis
dc.contributor.author
Soloneski, Sonia Maria Elsa
dc.date.available
2018-01-09T14:18:39Z
dc.date.issued
2014-08
dc.identifier.citation
Soloneski, Sonia Maria Elsa; Nikoloff, Noelia; Larramendy, Marcelo Luis; Comparative evaluation in vitro of the herbicide flurochloridone by cytokinesis-block micronucleus cytome and comet assays; John Wiley & Sons Inc; Environmental Toxicology; 29; 8; 8-2014; 884-892
dc.identifier.issn
1520-4081
dc.identifier.uri
http://hdl.handle.net/11336/32643
dc.description.abstract
The in-vitro effects of flurochloridone and its formulations Twin Pack Gold® (25% a.i.) and Rainbow® (25% a.i.) were evaluated in Chinese Hamster Ovary K1 (CHO-K1) cells. The cytokinesis-block micronucleus cytome (CBMN-cyt) and single-cell gel electrophoresis (SCGE) assays were used. The activities were tested within the range of final concentrations of 0.25–15 μg flurochloridone/mL. The results demonstrated that both the flurochloridone and Rainbow® were not able to induce micronuclei (MN). On the other hand, Twin Pack Gold® only increased the frequency of MN at 5 μg/mL. Furthermore, 10 and 15 μg/mL of both formulations resulted in a cellular cytotoxicity demonstrated by alterations in the nuclear division index and cellular death. SCGE assay appeared to be a more sensitive bioassay for detecting primary DNA strand breaks at lower concentrations of flurochloridone than MN did. A marked increase in the genetic damage index was observed when 5 and 15 μg/mL of both flurochloridone and Rainbow® but only when 15 μg/mL of Twin Pack Gold® were used. This is the first report demonstrating that flurochloridone and its two commercial formulations are able to induce single-strand DNA breaks in vitro on mammalian cells.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
John Wiley & Sons Inc
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Flurochloridone
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Cbmn-Cyt Assay
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Dna-Single Strands Breaks
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Cho-K1 Cells
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Commercial Formulations
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Otras Ciencias Biológicas
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Comparative evaluation in vitro of the herbicide flurochloridone by cytokinesis-block micronucleus cytome and comet assays
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-01-08T18:40:52Z
dc.journal.volume
29
dc.journal.number
8
dc.journal.pagination
884-892
dc.journal.pais
Estados Unidos
dc.journal.ciudad
New York
dc.description.fil
Fil: Nikoloff, Noelia. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina
dc.description.fil
Fil: Larramendy, Marcelo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina
dc.description.fil
Fil: Soloneski, Sonia Maria Elsa. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina
dc.journal.title
Environmental Toxicology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/tox.21816
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/tox.21816/abstract


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