Artículo
Detection of the Matrix Metalloproteinases MMP-2 and MMP-9 and Tissue Inhibitors of Metalloproteinases TIMP-1 and TIMP-2 in Llama (Lama glama) Oviduct
Fecha de publicación:
04/2014
Editorial:
Wiley
Revista:
Reproduction in Domestic Animals
ISSN:
0936-6768
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Matrix metalloproteinases (MMPs) and their inhibitors (TIMPs) are involved in several reproductive events like oocyte–spermatozoa interaction and semen liquefaction. In order to study their role in the llama oviductal reproductive process, MMP activity in oviductal fluid (OF) was assayed. Considering that llama genome sequences are partially known, a strategy to procure cDNA sequences of MMP-2, MMP-9, TIMP-1 and TIMP-2 was designed. Afterwards, their expression patterns in the different llama oviductal segments were assayed. Gelatine zymograms detected 62 and 94 kDa protease activities that matched MMP-2 and pro-MMP-9, respectively. Expression pattern analysis showed that MMP and TIMP mRNAs were present in ampulla, isthmus, utero-tubal junction (UTJ) and papilla. Altogether, these findings support the argument that MMPs/TIMPs are produced in the oviduct and secreted into the oviductal lumen. Our results encourage further studies to elucidate the role of these proteins in reproductive oviductal events.
Palabras clave:
Mmp
,
Oviducto
,
Llama
,
Timps
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(INSIBIO)
Articulos de INST.SUP.DE INVEST.BIOLOGICAS
Articulos de INST.SUP.DE INVEST.BIOLOGICAS
Citación
Apichela, Silvana Andrea; Miceli, Dora Cristina; Argañaraz, Martin Eduardo; Zampini, Renato; Detection of the Matrix Metalloproteinases MMP-2 and MMP-9 and Tissue Inhibitors of Metalloproteinases TIMP-1 and TIMP-2 in Llama (Lama glama) Oviduct; Wiley; Reproduction in Domestic Animals; 49; 3; 4-2014; 492-498
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