Artículo
In vitro plantlets regeneration of Schinopsis balansae (Anacardiaceae)
Sansberro, Pedro Alfonso
; Rey de Badaró, Hebe Yolanda
; Mroginski, Luis Amado
; Luna, Claudia Verónica
Fecha de publicación:
12/2003
Editorial:
Springer Berlin Heidelberg
Revista:
Trees
ISSN:
0931-1890
e-ISSN:
1432-2285
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Resumen
A protocol for the in vitro regeneration of rooted plants from nodal single bud segments of 10-year-old Schinopsis balansae trees was developed. Nodal segments were harvested from actively growing shoots of plants grown from seeds and maintained in pots under greenhouse conditions, and from epicormic shoots obtained by forced flushing of branches. Culture of nodal segments on nutrient medium containing the mineral salts and vitamins of Murashige and Skoog medium at 1/4 strength (1/4 MS), supplemented with 100 mg l−1 ascorbic acid, 3% sucrose, and 5–15 μM 6-benzyladenine resulted in regeneration of multiple shoots. Rooting of regenerated shoots was observed in 1/4 MS medium with vermiculite as the substrate and supplemented with 7.5 μM indolbutyric acid.
Palabras clave:
Schinopsis Balansae
,
Micropropagation
,
Nodal Explants
,
Tissue Culture
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(IBONE)
Articulos de INST.DE BOTANICA DEL NORDESTE (I)
Articulos de INST.DE BOTANICA DEL NORDESTE (I)
Citación
Sansberro, Pedro Alfonso; Rey de Badaró, Hebe Yolanda; Mroginski, Luis Amado; Luna, Claudia Verónica; In vitro plantlets regeneration of Schinopsis balansae (Anacardiaceae); Springer Berlin Heidelberg; Trees; 17; 6; 12-2003; 542-546
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