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Artículo

The Escherichia coli yjfP Gene Encodes a Carboxylesterase Involved in Sugar Utilization during Diauxie

Johns, Nat; Wrench, Algevis; Loto, Flavia del ValleIcon ; Valladares, Ricardo; Lorca, Graciela; Gonzalez, Claudio F.
Fecha de publicación: 12/2015
Editorial: Karger
Revista: Journal of Molecular Microbiology and Biotechnology
ISSN: 1464-1801
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Biológicas

Resumen

Background: Acetylation and efflux of carbohydrates during cellular metabolism is a well-described phenomenon associated with a detoxification process to prevent metabolic congestion. It is still unclear why cells discard important metabolizable energy sources in the form of acetylated compounds. Methods: We describe the purification and characterization of an approximately 28-kDa intracellular carboxylesterase (YjfP) and the analysis of gene and protein expression by qRT-PCR and Western blot. Results: qRT-PCR and Western blot, respectively, showed that yjfP is upregulated during the diauxic lag in cells growing with a mixture of glucose and lactose. The β-galactosidase activity in the ΔyjfP strain was both delayed and half the magnitude of that of the wild-type strain. YjfP-hyperproducing strains displayed a long lag phase when cultured with glucose and then challenged to grow with lactose or galactose as the sole carbon source. Conclusion: Our results suggest that YjfP controls the intracellular concentration of acetyl sugars by redirecting them to the main metabolic circuits. Instead of detoxification, we propose that sugar acetylation is utilized by the cell for protection and to prevent the metabolism of a necessary minimal intracellular sugar pool. Those sugars can eventually be exported as a side effect of these mechanisms.
Palabras clave: Carbohydrate Metabolism , Carboxylesterases , Diauxie , Escherichia Coli , Yjfp
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/30154
DOI: https://dx.doi.org/10.1159/000442819
URL: https://www.karger.com/Article/FullText/442819
Colecciones
Articulos(PROIMI)
Articulos de PLANTA PILOTO DE PROC.IND.MICROBIOLOGICOS (I)
Citación
Johns, Nat; Wrench, Algevis; Loto, Flavia del Valle; Valladares, Ricardo; Lorca, Graciela; et al.; The Escherichia coli yjfP Gene Encodes a Carboxylesterase Involved in Sugar Utilization during Diauxie; Karger; Journal of Molecular Microbiology and Biotechnology; 25; 6; 12-2015; 412-422
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