Artículo
Surface modification on poly (EGDMA-co-HEMA) synthetic matrices to be used as specific adsorbents
Fecha de publicación:
12/2005
Editorial:
Elsevier
Revista:
Polymer
ISSN:
0032-3861
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The preparation and modification of ethylene glycol dimethacrylate/2-hydroxyethyl methacrylate copolymers [poly (EGDMA-co-HEMA)] were carried out to study their properties on Immobilized Metal Ion Affinity Chromatography (IMAC). For that purpose, the adsorption–desorption assays of a phosphoprotein, ovalbumin (OVA) on different ion-containing adsorbents were investigated. The metal ion-complexing agent used was iminodiacetic acid (IDA) and the metal ions chelated were Al3+ or Fe3+. The efficiency on both modification reactions and OVA retention was analysed taking into account the pore volumes (Vp) and specific surface area (Ss) values in macropores zones and equilibrium volume-swelling ratio (qv) from base matrices and final ion-containing supports. In general, Fe3+-containing products resulted more efficient adsorbents in the OVA retention and although the adsorption assays on Fe3+ or Al3+-containing supports were fast, the complete and faster OVA desorption was reached when Al3+-containing matrix was used.
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Identificadores
Colecciones
Articulos(CCT - CORDOBA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Articulos(IMBIV)
Articulos de INST.MULTIDISCIPL.DE BIOLOGIA VEGETAL (P)
Articulos de INST.MULTIDISCIPL.DE BIOLOGIA VEGETAL (P)
Citación
Gomez, Cesar Gerardo; Alvarez Igarzabal, Cecilia Ines; Strumia, Miriam Cristina; Surface modification on poly (EGDMA-co-HEMA) synthetic matrices to be used as specific adsorbents; Elsevier; Polymer; 46; 17; 12-2005; 6300-6307
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