Culture studies on the mycobiont isolated from Parmotrema reticulatum (Taylor) Choisy: Metabolite production under different conditions

Abstract

A strain of the lichen mycobiont isolated from Parmotrema reticulatum was cultured axenically on different media. The morphology, anatomy, growth of the colonies, and metabolite production were studied. The iisolated fungal colonies developed well and showed aremarkable morphogenetic capacity on most of the assayedsolid media, e.g., malt extract 2%-yeast extract 0.2% (MEYE), malt extract 1%-yeast extract 0.4%-sucrose 10% (MY10), and the original Lilly & Barnett medium (LB). Theidentity of the isolated fungus was confirmed by its ITS rDNA-sequence. Atranorin, the major cortical lichen depside,was produced when the colonies were grown over 5