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dc.contributor.author
Dematteis, Andrea
dc.contributor.author
Miranda, Sabrina Desiree
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Novella, María de Lourdes
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Maldonado, Cristina Alicia
dc.contributor.author
Ponce, Rubén Hugo
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Maldera, Julieta Antonella
dc.contributor.author
Cuasnicu, Patricia Sara
dc.contributor.author
Coronel, Carlos Enrique
dc.date.available
2017-10-10T15:09:34Z
dc.date.issued
2008
dc.identifier.citation
Dematteis, Andrea; Miranda, Sabrina Desiree; Novella, María de Lourdes; Maldonado, Cristina Alicia; Ponce, Rubén Hugo; et al.; Rat caltrin protein modulates the acrosomal exocytosis during sperm capacitation; Society for the Study of Reproduction; Biology of Reproduction; 79; 3; -1-2008; 493-500
dc.identifier.issn
0006-3363
dc.identifier.uri
http://hdl.handle.net/11336/26330
dc.description.abstract
Caltrin is a small and basic protein of the seminal vesicle secretion that inhibits sperm calcium uptake. The influence of rat caltrin on sperm physiological processes related to fertilizing competence was studied by examining its effect on 1) spontaneous acrosomal exocytosis, 2) protein tyrosine phosphorylation, and 3) sperm-egg interaction. Results show that the presence of caltrin during in vitro capacitation both reduced the rate of spontaneous acrosomal exocytosis without altering the pattern of protein tyrosine phosphorylation, and enhanced the sperm ability to bind to the zona pellucida (ZP). The significantly higher proportion of sperm with intact acrosome observed in the presence of caltrin was accompanied by a strong inhibition in the acrosomal hyaluronidase release. Enhancement of sperm-ZP binding was evident by the increase in the percentage of eggs with bound spermatozoa as well as in the number of bound sperm per egg. Similar results were obtained when the assays were performed using spermatozoa preincubated with caltrin and then washed to remove the unbound protein, indicating that the sperm-bound caltrin was the one involved in both acrosomal exocytosis inhibition and sperm-ZP binding enhancement. Caltrin bound to the sperm head was partially released during the acrosomal exocytosis induced by Ca-ionophore A23187. Indirect immunofluorescence and immunoelectron microscopy studies revealed that caltrin molecules distributed on the dorsal sperm surface disappeared after ionophore exposure, whereas those on the ventral region remained in this localization after the treatment. The present data suggest that rat caltrin molecules bound to the sperm head during ejaculation prevent the occurrence of the spontaneous acrosomal exocytosis along the female reproductive tract. Consequently, more competent spermatozoa with intact and functional acrosome would be available in the oviduct to participate in fertilization.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Society for the Study of Reproduction
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Acrosome Reaction
dc.subject
Calcium
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Male Reproductive Tract
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Sperm Capacitation
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Male Reproductive Tract
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Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Rat caltrin protein modulates the acrosomal exocytosis during sperm capacitation
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-10-04T16:55:28Z
dc.identifier.eissn
1529-7268
dc.journal.volume
79
dc.journal.number
3
dc.journal.pagination
493-500
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Nueva York
dc.conicet.avisoEditorial
This is a pre-copyedited, author-produced version of an article accepted for publication in Biology of Reproduction following peer review. The version of record Andrea Dematteis, Sabrina D. Miranda, Maria L. Novella, Cristina Maldonado, Ruben H. Ponce, Julieta A. Maldera, Patricia S. Cuasnicu, Carlos E. Coronel; Rat Caltrin Protein Modulates the Acrosomal Exocytosis During Sperm Capacitation, Biology of Reproduction, Volume 79, Issue 3, 1 September 2008, Pages 493–500 is available online at: https://doi.org/10.1095/biolreprod.107.067538
dc.description.fil
Fil: Dematteis, Andrea. Universidad Nacional de Córdoba; Argentina
dc.description.fil
Fil: Miranda, Sabrina Desiree. Universidad Nacional de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
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Fil: Novella, María de Lourdes. Universidad Nacional de Córdoba; Argentina
dc.description.fil
Fil: Maldonado, Cristina Alicia. Universidad Nacional de Córdoba. Facultad de Medicina. Centro de Microscopia Electronica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
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Fil: Ponce, Rubén Hugo. Universidad Nacional de Córdoba; Argentina
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Fil: Maldera, Julieta Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil
Fil: Cuasnicu, Patricia Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil
Fil: Coronel, Carlos Enrique. Universidad Nacional de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Biology of Reproduction
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/biolreprod/article-lookup/doi/10.1095/biolreprod.107.067538
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1095/biolreprod.107.067538
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/pmid/18550793
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