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dc.contributor.author
Blanco, Federico Carlos
dc.contributor.author
Bianco, María Verónica
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Garbaccio, Sergio
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Meikle, Virginia
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Gravisaco, María José
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Montenegro, Valeria Noely
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Alfonseca, Edgar
dc.contributor.author
Singh, Mahavir
dc.contributor.author
Barandiaran, Soledad
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Canal, Ana María
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Vagnoni, Lucas
dc.contributor.author
Buddle, Bryce Malcom
dc.contributor.author
Bigi, Fabiana
dc.contributor.author
Cataldi, Ángel Adrián
dc.date.available
2017-09-12T16:26:52Z
dc.date.issued
2013-03
dc.identifier.citation
Blanco, Federico Carlos; Bianco, María Verónica; Garbaccio, Sergio; Meikle, Virginia; Gravisaco, María José; et al.; Mycobacterium bovis Δmce2 double deletion mutant protects cattle against challenge with virulent M. bovis; Elsevier; Tuberculosis (Edinb); 93; 3; 3-2013; 363-372
dc.identifier.issn
1472-9792
dc.identifier.uri
http://hdl.handle.net/11336/24002
dc.description.abstract
A Mycobacterium bovis strain deleted in mce2A and mce2B genes (M. bovis Δmce2) was tested as an experimental vaccine in cattle challenged with a virulent M. bovis strain. Three-and-a-half-month old calves (n = 5 to 6 per group) were vaccinated and challenged with a virulent strain of M. bovis by the intratracheal route 9 weeks after vaccination. A non-vaccinated group and a group vaccinated with BCG were included as controls. Blood samples were collected to measure IFN-γ by an interferon-gamma release assay (IGRA), cytometry and cytokine responses of bovine purified protein derivative (PPD) restimulated peripheral blood mononuclear cells (PBMCs). The IGRA test showed IFN-γ values similar to pre-vaccination except for the animals vaccinated with M. bovis Δmce2, where a significant increase was observed at 30 days post-vaccination. The expression of IL-2R on CD4(+) cells in response to PPD from the animals vaccinated with Δmce2 increased at 15 days post-vaccination compared to cells from non-vaccinated group. Vaccination of cattle with M. bovis Δmce2 induced the highest (P < 0.05) expression of IFN-γ and IL-17 mRNA upon PPD stimulation of PBMCs compared to vaccination with BCG or that for the non-vaccinated group. There was a weak positive correlation between the production of these proinflammatory cytokines post-vaccination and reduced pathology scores post-challenge. The animals were euthanized and necropsied 100 days after challenge. The group vaccinated with M. bovis Δmce2 displayed a significantly lower histopathological score for lesions in lungs and pulmonary lymph nodes than for the other groups (P < 0.05). A marked positive reaction to tuberculin intradermal test was observed post-vaccination in animals vaccinated with M. bovis Δmce2 compared to those vaccinated with BCG or the non-vaccinated group. In contrast, after challenge, non-vaccinated animals had greater skin test responses than the vaccinated animals. In summary, M. bovis Δmce2 is a promising vaccine candidate to control M. bovis pathogenesis in cattle.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Bovine Tuberculosis
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Mycobacterium Bovis
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Live Vaccine
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Mce
dc.title
Mycobacterium bovis Δmce2 double deletion mutant protects cattle against challenge with virulent M. bovis
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-09-08T20:22:34Z
dc.journal.volume
93
dc.journal.number
3
dc.journal.pagination
363-372
dc.journal.pais
Países Bajos
dc.journal.ciudad
Ámsterdam
dc.description.fil
Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Bianco, María Verónica. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
dc.description.fil
Fil: Garbaccio, Sergio. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
dc.description.fil
Fil: Meikle, Virginia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
dc.description.fil
Fil: Gravisaco, María José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
dc.description.fil
Fil: Montenegro, Valeria Noely. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Alfonseca, Edgar. Universidad Nacional Autónoma de México; México
dc.description.fil
Fil: Singh, Mahavir. Lionex; Alemania
dc.description.fil
Fil: Barandiaran, Soledad. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Canal, Ana María. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
dc.description.fil
Fil: Vagnoni, Lucas. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina
dc.description.fil
Fil: Buddle, Bryce Malcom. Hopkirk Research Institute; Nueva Zelanda
dc.description.fil
Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Cataldi, Ángel Adrián. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Tuberculosis (Edinb)
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.tube.2013.02.004
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1472979213000127
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