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dc.contributor.author
Borroni, Maria Virginia
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dc.contributor.author
Barrantes, Francisco Jose
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dc.date.available
2024-04-18T10:32:16Z
dc.date.issued
2010
dc.identifier.citation
Cholesterol levels determine AChR endocytic route in CHO-K1/A5 cells; Eukaryotic Lipids;Ttreasure of regulatory information; Spetses; Grecia; 2010; 13-13
dc.identifier.uri
http://hdl.handle.net/11336/233376
dc.description.abstract
Stability of the nicotinic acetylcholine receptor (AChR) at the cell surface is critical to the correct functioning of the cholinergic synapse. Cholesterol (Chol) is an essential lipid that modulates AChR levels at the plasmalemma and ion translocation. We have studied the endocytosis of AChR in CHO-K1/A5 cells, a Chinese hamster ovary (CHO) cell line heterologously expressing murine muscle adult-type receptor under different Chol membrane content. Contrary to the norm, endocytosis of cell-surface AChR is accelerated by membrane Chol depletion via a hitherto unknown mechanism. This acceleration is no longer operative when membrane Chol levels are restored. We explored the possible mechanism involved in receptor loss in Choldepleted cells (Chol-). Under such conditions the AChR is internalized by a ligand-, clathrin- and dynaminindependent mechanism, which does not involve the presence of the AChR-associated protein rapsyn. The small GTPase Rac1 is required: expression of a dominant negative form of Rac1, Rac1N17, abrogates receptor endocytosis. At variance with the endocytic pathway in control CHO cells, the accelerated AChR internalization proceeds even upon disruption of the actin cytoskeleton and does not depend on the cytoskeleton-associated inositol lipid PI(4,5)P2; its sequestration by the PH domain of phospholipase C does not alter endocytosis. AChR internalization under Chol- conditions is furthermore found to require the activity of Arf6 and its effectors Rac1 and phospholipase D. Thus, membrane Chol appears to act as a key homeostatic regulator of cell-surface receptor levels, determining the rate and mechanism of AChR endocytosis
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Federation of European Biochemical Societies
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
NICOTINIC RECEPTOR
dc.subject
CHOLESTEROL
dc.subject
LIPID DOMAINS
dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Cholesterol levels determine AChR endocytic route in CHO-K1/A5 cells
dc.type
info:eu-repo/semantics/publishedVersion
dc.type
info:eu-repo/semantics/conferenceObject
dc.type
info:ar-repo/semantics/documento de conferencia
dc.date.updated
2024-03-25T15:42:29Z
dc.journal.pagination
13-13
dc.journal.pais
Reino Unido
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dc.journal.ciudad
Cambridge
dc.description.fil
Fil: Borroni, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
dc.description.fil
Fil: Barrantes, Francisco Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://web.science.uu.nl/archive/Spetses2010/posters.pdf
dc.conicet.rol
Autor
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dc.conicet.rol
Autor
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dc.coverage
Internacional
dc.type.subtype
Workshop
dc.description.nombreEvento
Eukaryotic Lipids;Ttreasure of regulatory information
dc.date.evento
2010-06-19
dc.description.ciudadEvento
Spetses
dc.description.paisEvento
Grecia
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dc.type.publicacion
Book
dc.description.institucionOrganizadora
Federation of European Biochemical Societies
dc.description.institucionOrganizadora
International Union for Biochemistry and Molecular Biology
dc.source.libro
Eukaryotic Lipids;treasure of regulatory information
dc.date.eventoHasta
2010-01-24
dc.type
Workshop
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