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dc.contributor.author
Borroni, Maria Virginia  
dc.contributor.author
Barrantes, Francisco Jose  
dc.date.available
2024-04-18T10:32:16Z  
dc.date.issued
2010  
dc.identifier.citation
Cholesterol levels determine AChR endocytic route in CHO-K1/A5 cells; Eukaryotic Lipids;Ttreasure of regulatory information; Spetses; Grecia; 2010; 13-13  
dc.identifier.uri
http://hdl.handle.net/11336/233376  
dc.description.abstract
Stability of the nicotinic acetylcholine receptor (AChR) at the cell surface is critical to the correct functioning of the cholinergic synapse. Cholesterol (Chol) is an essential lipid that modulates AChR levels at the plasmalemma and ion translocation. We have studied the endocytosis of AChR in CHO-K1/A5 cells, a Chinese hamster ovary (CHO) cell line heterologously expressing murine muscle adult-type receptor under different Chol membrane content. Contrary to the norm, endocytosis of cell-surface AChR is accelerated by membrane Chol depletion via a hitherto unknown mechanism. This acceleration is no longer operative when membrane Chol levels are restored. We explored the possible mechanism involved in receptor loss in Choldepleted cells (Chol-). Under such conditions the AChR is internalized by a ligand-, clathrin- and dynaminindependent mechanism, which does not involve the presence of the AChR-associated protein rapsyn. The small GTPase Rac1 is required: expression of a dominant negative form of Rac1, Rac1N17, abrogates receptor endocytosis. At variance with the endocytic pathway in control CHO cells, the accelerated AChR internalization proceeds even upon disruption of the actin cytoskeleton and does not depend on the cytoskeleton-associated inositol lipid PI(4,5)P2; its sequestration by the PH domain of phospholipase C does not alter endocytosis. AChR internalization under Chol- conditions is furthermore found to require the activity of Arf6 and its effectors Rac1 and phospholipase D. Thus, membrane Chol appears to act as a key homeostatic regulator of cell-surface receptor levels, determining the rate and mechanism of AChR endocytosis  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Federation of European Biochemical Societies  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
NICOTINIC RECEPTOR  
dc.subject
CHOLESTEROL  
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LIPID DOMAINS  
dc.subject.classification
Bioquímica y Biología Molecular  
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Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Cholesterol levels determine AChR endocytic route in CHO-K1/A5 cells  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.type
info:eu-repo/semantics/conferenceObject  
dc.type
info:ar-repo/semantics/documento de conferencia  
dc.date.updated
2024-03-25T15:42:29Z  
dc.journal.pagination
13-13  
dc.journal.pais
Reino Unido  
dc.journal.ciudad
Cambridge  
dc.description.fil
Fil: Borroni, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina  
dc.description.fil
Fil: Barrantes, Francisco Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://web.science.uu.nl/archive/Spetses2010/posters.pdf  
dc.conicet.rol
Autor  
dc.conicet.rol
Autor  
dc.coverage
Internacional  
dc.type.subtype
Workshop  
dc.description.nombreEvento
Eukaryotic Lipids;Ttreasure of regulatory information  
dc.date.evento
2010-06-19  
dc.description.ciudadEvento
Spetses  
dc.description.paisEvento
Grecia  
dc.type.publicacion
Book  
dc.description.institucionOrganizadora
Federation of European Biochemical Societies  
dc.description.institucionOrganizadora
International Union for Biochemistry and Molecular Biology  
dc.source.libro
Eukaryotic Lipids;treasure of regulatory information  
dc.date.eventoHasta
2010-01-24  
dc.type
Workshop