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dc.contributor.author
Doni, Davide
dc.contributor.author
Cavallari, Eva
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Noguera, Martín Ezequiel
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Gentili, Hernan Gustavo
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Cavion, Federica
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Parisi, Gustavo Daniel
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Fornasari, Maria Silvina
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Sartori, Geppo
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Santos, Javier
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Bellanda, Massimo
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Carbonera, Donatella
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Costantini, Paola
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Bortolus, Marco
dc.date.available
2024-04-03T11:57:54Z
dc.date.issued
2024-02
dc.identifier.citation
Doni, Davide; Cavallari, Eva; Noguera, Martín Ezequiel; Gentili, Hernan Gustavo; Cavion, Federica; et al.; Searching for Frataxin Function: Exploring the Analogy with Nqo15, the Frataxin-like Protein of Respiratory Complex I from Thermus thermophilus; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 25; 3; 2-2024; 1-18
dc.identifier.issn
1422-0067
dc.identifier.uri
http://hdl.handle.net/11336/231759
dc.description.abstract
Nqo15 is a subunit of respiratory complex I of the bacterium Thermus thermophilus, withstrong structural similarity to human frataxin (FXN), a protein involved in the mitochondrial diseaseFriedreich’s ataxia (FRDA). Recently, we showed that the expression of recombinant Nqo15 canameliorate the respiratory phenotype of FRDA patients’ cells, and this prompted us to furthercharacterize both the Nqo15 solution’s behavior and its potential functional overlap with FXN, usinga combination of in silico and in vitro techniques. We studied the analogy of Nqo15 and FXN byperforming extensive database searches based on sequence and structure. Nqo15’s folding andflexibility were investigated by combining nuclear magnetic resonance (NMR), circular dichroism,and coarse-grained molecular dynamics simulations. Nqo15’s iron-binding properties were studiedusing NMR, fluorescence, and specific assays and its desulfurase activation by biochemical assays. Wefound that the recombinant Nqo15 isolated from complex I is monomeric, stable, folded in solution,and highly dynamic. Nqo15 does not share the iron-binding properties of FXN or its desulfuraseactivation function.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Molecular Diversity Preservation International
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
NqO15
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frataxin
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Friedreich Ataxia
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Thermus thermophilus
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Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Searching for Frataxin Function: Exploring the Analogy with Nqo15, the Frataxin-like Protein of Respiratory Complex I from Thermus thermophilus
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2024-03-19T14:14:26Z
dc.journal.volume
25
dc.journal.number
3
dc.journal.pagination
1-18
dc.journal.pais
Suiza
dc.description.fil
Fil: Doni, Davide. Università di Padova; Italia
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Fil: Cavallari, Eva. Università di Padova; Italia
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Fil: Noguera, Martín Ezequiel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biociencias, Biotecnología y Biología Traslacional.; Argentina. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
dc.description.fil
Fil: Gentili, Hernan Gustavo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biociencias, Biotecnología y Biología Traslacional.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
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Fil: Cavion, Federica. Università di Padova; Italia
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Fil: Parisi, Gustavo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; Argentina
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Fil: Fornasari, Maria Silvina. Universidad Nacional de Quilmes; Argentina. Universidad Nacional de Quilmes; Argentina
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Fil: Sartori, Geppo. Università di Padova; Italia
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Fil: Santos, Javier. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biociencias, Biotecnología y Biología Traslacional.; Argentina
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Fil: Bellanda, Massimo. Università di Padova; Italia
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Fil: Carbonera, Donatella. Università di Padova; Italia
dc.description.fil
Fil: Costantini, Paola. Università di Padova; Italia
dc.description.fil
Fil: Bortolus, Marco. Università di Padova; Italia
dc.journal.title
International Journal of Molecular Sciences
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/25/3/1912
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3390/ijms25031912
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